caa a22 4500 397557590 CHVBK 20180308164807.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021304 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021304 Effect of Exogenous Decorin on Cell Morphology and Attachment of Decorin-Deficient Fibroblasts [Elektronische Daten] [Jianguo Gu, Yoshinao Wada] We have reported deficient expression of decorin on skin fibroblasts from a patient with carbohydrate-deficient glycoprotein syndrome type I [Gu, J. and Wada, Y. (1995)J. Biochem. 117, 1276-1279]. The characteristics of fibroblasts from this patient included increased cell spreading and reduced proliferation. We analyzed the expression of other extracellular matrix proteins by Western and Northern blot analyses, and found that adhesion molecules, fibronectin, and type I collagen, were increased, whereas an anti-adhesion molecule, tenascin, was decreased, like decorin. Subsequently, decorin was purified from bovine tendons, and cultured with these fibroblasts in fibronectin-depleted culture medium. Exogenous decorin inhibited cell attachment to a plastic culture dish in a dose-dependent manner, while dermatan sulfate did not. The cell morphology was markedly normalized by decorin, but proliferation was not restored. These findings suggest that decorin exhibits an anti-adhesion property in a fibroblast culture system and that the deficiency is responsible for the morphological change observed in this patient's fibroblasts. © by the Journal of Biochemistry Regular Papers nationallicence adhesion molecule nationallicence cell attachment nationallicence cell spreading nationallicence decorin nationallicence fibroblast nationallicence Gu Jianguo Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 590-02 aut Wada Yoshinao Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 590-02 aut The Journal of Biochemistry Oxford University Press 119/4(1996-04), 743-748 0021-924X 119:4<743 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021304 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021304 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gu Jianguo Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 590-02 aut NATIONALLICENCE 700 1- Wada Yoshinao Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 590-02 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/4(1996-04), 743-748 0021-924X 119:4<743 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford