caa a22 4500 397557655 CHVBK 20180308164807.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021328 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021328 Cell Cycle-Dependent Phosphorylation of Smooth Muscle Myosin Light Chain in Sea Urchin Egg Extracts [Elektronische Daten] [Masanori Mishima, Issei Mabuchi] We studied enzymatic activities in sea urchin egg extracts that phosphorylate myosin regulatory light chain (MRLC) from chicken gizzard smooth muscle. The activity in the presence of EGTA showed cell cycle-dependent changes similar to that of hi stone HI kinase, namely, it peaked shortly before cleavage, while that in the presence of Ca2+ ions did not show significant change during division cycle. Phosphopeptide mapping revealed that both the sites phosphorylatable by smooth muscle myosin light chain kinase (MLCK sites) and the sites phosphorylatable by protein kinase C (PKC sites) were phosphorylated in the presence or absence of Ca2+ ions. By analyses using an inhibitor of cdc2 kinase, butyro-lactone-I, and ion exchange column chromatography, at least three kinases were detected as kinases that phosphorylate MRLC in vitro. These kinases phosphorylated distinct sites on MRLC. The first one, which phosphorylated the PKC sites, was identified as cdc2 kinase. The second one phosphorylated the MLCK sites in the absence of Ca2+ ions. The third one phosphorylated unknown sites. Possible implication of these activities in regulation of cytokinesis is discussed. © by the Journal of Biochemistry Regular Papers nationallicence cell division nationallicence histone HI kinase nationallicence myosin light chain nationallicence phosphorylation nationallicence sea urchin nationallicence Mishima Masanori Department of Biology, School of Arts and Sciences, The University of Tokyo Kornaba, Meguro-ku, Tokyo 153 aut Mabuchi Issei Department of Biology, School of Arts and Sciences, The University of Tokyo Kornaba, Meguro-ku, Tokyo 153 aut The Journal of Biochemistry Oxford University Press 119/5(1996-05), 906-913 0021-924X 119:5<906 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021328 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021328 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mishima Masanori Department of Biology, School of Arts and Sciences, The University of Tokyo Kornaba, Meguro-ku, Tokyo 153 aut NATIONALLICENCE 700 1- Mabuchi Issei Department of Biology, School of Arts and Sciences, The University of Tokyo Kornaba, Meguro-ku, Tokyo 153 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/5(1996-05), 906-913 0021-924X 119:5<906 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford