caa a22 4500 397557671 CHVBK 20180308164807.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021338 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021338 Structural Organization and Promoter Activity of the Human Ryuddcan Gene [Elektronische Daten] [Akira Takagi, Tetsuhito Kojima, Shinobu Tsuzuki, Akira Katsumi, Tomio Yamazaki, Isamu Sugiura, Motohiro Hamaguchi, Hidehiko Saito] To better understand the regulation of ryudocan (syndecan-4) expression, we have determined the structural organization of the human ryudocan gene. The human ryudocan gene extends approximately 24 kilobases and is divided into five exons, which appear to be conserved in syndecan family members. Exon I encodes the signal peptide; exons II-IV, the extracellular domain; and exon V, the transmembrane and cytoplasmic domains, which are highly homologous among syndecan family members. Primer extension analysis showed that human ryudocan gene had a single transcription initiation site, located 3 bases upstream from the described cDNA [Kojima et al. (1993) BBRC 190, 814-822]. The 5′-flanking sequences of human ryudocan gene contain a TATA-like sequence as well as a variety of other potential binding sites for transcription factors, including Spl, Ap-2, NF-kB, E-alpha H box, H4TF-2, and LBP-1, and were capable of functioning as a promoter. The determination of the human ryudocan gene structure will allow elucidation of constitutive, cell-specific, tissue-specific, and developmentally regulated expression. © by the Journal of Biochemistry Regular Papers nationallicence core protein nationallicence gene organization nationallicence heparan sulfate proteoglycan nationallicence human ryudocan nationallicence promoter function nationallicence Takagi Akira First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut Kojima Tetsuhito First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut Tsuzuki Shinobu First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut Katsumi Akira First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut Yamazaki Tomio First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut Sugiura Isamu First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut Hamaguchi Motohiro First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut Saito Hidehiko First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut The Journal of Biochemistry Oxford University Press 119/5(1996-05), 979-984 0021-924X 119:5<979 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021338 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021338 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takagi Akira First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut NATIONALLICENCE 700 1- Kojima Tetsuhito First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut NATIONALLICENCE 700 1- Tsuzuki Shinobu First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut NATIONALLICENCE 700 1- Katsumi Akira First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut NATIONALLICENCE 700 1- Yamazaki Tomio First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut NATIONALLICENCE 700 1- Sugiura Isamu First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut NATIONALLICENCE 700 1- Hamaguchi Motohiro First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut NATIONALLICENCE 700 1- Saito Hidehiko First Department of Internal Medicine, Nagoya University School of Medicine Nagoya 466 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/5(1996-05), 979-984 0021-924X 119:5<979 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford