caa a22 4500 39755768X CHVBK 20180308164807.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021340 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021340 Substrate Specificity of Bovine Liver Cytosolic Neutral α-Mannosidase Activated by Co2+ [Elektronische Daten] [Masashi Kumano, Kaoru Omichi, Sumihiro Hase] A cytosolic neutral α-mannosidase was purified from bovine liver. Its molecular weight was found to be 500,000 on gel filtration. The activity of the enzyme toward Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAc-PA was increased 26-fold by preincubation with 1 mM Co2+. Manα1-6(Manα1-3)Manβ1-4GlcNAc was hydrolyzed by the enzyme to Manα1-3Manα1-6(Manα1-3)Manβ1-4GlcNAc, which was further hydrolyzed to Manα1-6(Manα1-3)Manβ1-4GlcNAc. The rate of hydrolysis was 15-fold greater than that of Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAc. This substrate specificity suggested that the enzyme could be involved in the degradation of oligomannose-type sugar chains with one GlcNAc residue released from glycoproteins by endo- β-N-acetylglucosaminidase, and supported a pathway for glycoprotein catabolism via oligomannosyl glycans with one GlcNAc residue proposed on the basis of an earlier study on a cytosolic neutral α-mannosidase from Japanese quail oviduct [Oku, H. and Hase, S. (1991) J. Biochem 110, 982-989]. © by the Journal of Biochemistry Regular Papers nationallicence α-mannosidase nationallicence cytosol nationallicence oligomannosyl glycan nationallicence substrate specificity nationallicence Kumano Masashi Department of Chemistry, Osaka University College of Science, Toyonaka, Osaka 560 aut Omichi Kaoru Department of Chemistry, Osaka University College of Science, Toyonaka, Osaka 560 aut Hase Sumihiro Department of Chemistry, Osaka University College of Science, Toyonaka, Osaka 560 aut The Journal of Biochemistry Oxford University Press 119/5(1996-05), 991-997 0021-924X 119:5<991 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021340 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021340 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kumano Masashi Department of Chemistry, Osaka University College of Science, Toyonaka, Osaka 560 aut NATIONALLICENCE 700 1- Omichi Kaoru Department of Chemistry, Osaka University College of Science, Toyonaka, Osaka 560 aut NATIONALLICENCE 700 1- Hase Sumihiro Department of Chemistry, Osaka University College of Science, Toyonaka, Osaka 560 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/5(1996-05), 991-997 0021-924X 119:5<991 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford