caa a22 4500 39755771X CHVBK 20180308164807.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021321 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021321 β-Adrenergic Receptors in Rat Fat Cells and Their Relationship with Lipolysis [Elektronische Daten] [Hiromichi Okuda, Chie Morimoto, Takahiro Tsujita] Norepinephrine stimulated lipolysis in rat fat cells while (—) -alprenolol completely inhibited this lipolysis. (—)-Alprenolol competed for (—)-[3H]dihydroalprenolol(DHA) binding sites on rat fat cells. The specific (—)-[3H]DHA binding sites identified by competition with (—)-alprenolol were found to be transferred to the solubilized supernatant during preparation of endogenous lipid droplets from the fat cells. Although the lipid droplets did not exhibit specific (—)-[3H]DHA binding, norepinephrine induced lipolysis in a cell-free system consisting of the lipid droplets and hormone-sensitive lipase (HSL). Norepinephrine-induced lipolysis in the cell-free system was inhibited by pro-pranolol and (—)-alprenolol, but not by phenoxybenzamine. The lipolytic action of norepinephrine and the anti-lipolytic actions of propranolol and (—)-alprenolol disappeared after sonication of the lipid droplets in the cell-free system. These results suggest that the adrenergic receptor concerned with lipolysis in fat cells may not be a specific (—)- [3H]DHA binding site, but may be closely related to the lipid droplets. © by the Journal of Biochemistry Regular Papers nationallicence fat cell nationallicence lipid droplet nationallicence lipolysis nationallicence specific binding nationallicence Okuda Hiromichi 2nd Department of Medical Biochemistry, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-02 aut Morimoto Chie 2nd Department of Medical Biochemistry, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-02 aut Tsujita Takahiro 2nd Department of Medical Biochemistry, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-02 aut The Journal of Biochemistry Oxford University Press 119/5(1996-05), 852-856 0021-924X 119:5<852 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021321 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021321 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okuda Hiromichi 2nd Department of Medical Biochemistry, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-02 aut NATIONALLICENCE 700 1- Morimoto Chie 2nd Department of Medical Biochemistry, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-02 aut NATIONALLICENCE 700 1- Tsujita Takahiro 2nd Department of Medical Biochemistry, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-02 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/5(1996-05), 852-856 0021-924X 119:5<852 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford