caa a22 4500 397557744 CHVBK 20180308164807.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021333 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021333 Effect of Hepatocyte Growth Factor/Scatter Factor on Lipogenesis in Adult Rat Hepatocytes in Primary Culture [Elektronische Daten] [Qing Shao, Naokatu Arakaki, Tomokazu Ohnishi, Osamu Nakamura, Yasushi Daikuhara] Hepatocyte growth factor (HGF)/scatter factor is known to be the most potent mitogen for hepatocytes. In this paper, we report that lipogenesis in primary cultured rat hepatocytes treated with 10 ng/ml of recombinant human HGF (rhHGF) for 24 h was stimulated, as measured by the incorporation of 3H2O into long-chain fatty acids, to more than twice as much as the control. Insulin (0.1 μM) was more effective than rhHGF but rhHGF did not show an additive or synergistic effect when added to insulin. We also showed that treatment with rhHGF increased the activities of glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme, key enzymes which supply NADPH for lipogenesis, and acetyl-CoA carbox-ylase, the rate-limiting enzyme of lipogenesis. The increase in G6PDH and acetyl-CoA carboxylase activities was accompanied by increases in the levels of mRNA for the enzymes. These results suggest that HGF is involved in liver regeneration not only by stimulation of cell proliferation but also by acceleration of differentiation of hepatocytes. © by the Journal of Biochemistry Regular Papers nationallicence acetyl-CoA carboxylase nationallicence cell differentiation nationallicence hepatocyte nationallicence hepatocyte growth factor/scatter factor nationallicence lipogenesis nationallicence Shao Qing Department of Biochemistry, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, Kagoshima 890 aut Arakaki Naokatu Department of Biochemistry, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, Kagoshima 890 aut Ohnishi Tomokazu Department of Biochemistry, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, Kagoshima 890 aut Nakamura Osamu Department of Biochemistry, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, Kagoshima 890 aut Daikuhara Yasushi Department of Biochemistry, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, Kagoshima 890 aut The Journal of Biochemistry Oxford University Press 119/5(1996-05), 940-946 0021-924X 119:5<940 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021333 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021333 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shao Qing Department of Biochemistry, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, Kagoshima 890 aut NATIONALLICENCE 700 1- Arakaki Naokatu Department of Biochemistry, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, Kagoshima 890 aut NATIONALLICENCE 700 1- Ohnishi Tomokazu Department of Biochemistry, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, Kagoshima 890 aut NATIONALLICENCE 700 1- Nakamura Osamu Department of Biochemistry, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, Kagoshima 890 aut NATIONALLICENCE 700 1- Daikuhara Yasushi Department of Biochemistry, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, Kagoshima 890 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/5(1996-05), 940-946 0021-924X 119:5<940 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford