caa a22 4500 397557760 CHVBK 20180308164807.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021332 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021332 The Existence of Thyroid Hormone Responsive Element, TRE, Was Confirmed in the First Intron of Rat α1 -Acid Glycoprotein Gene [Elektronische Daten] [Torn Matsukawa, Masatoshi Kitagawa, Kazuhiko Katayama, Yukiko Nagai, Takayuki Hayashi, Hideyo Yasuda, Yoshiki Ohba] A set of acute-phase proteins was found to have been induced in rat after triiodothyronine (T3) treatment. To study the mechanism of gene activation by T3, the rat gene of α1,-acid glycoprotein (AGP), one of the major acute-phase proteins, was cloned and analyzed. CAT assay identified a thyroid hormone responsive element (TRE) in the first intron of the AGP gene and showed that mutations introduced into the TRE region destroyed the TRE activity. Gel shift assay and methylation interference assay showed that the thyroid hormone receptor bound to the TRE. The TRE identified here had a palindromic structure and was highly homologous to other known TREs. © by the Journal of Biochemistry Regular Papers nationallicence α1-acid glycoprotein nationallicence acute-phase proteins nationallicence gene expression nationallicence thyroid hormone nationallicence TRE nationallicence Matsukawa Torn Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut Kitagawa Masatoshi Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut Katayama Kazuhiko Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut Nagai Yukiko Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut Hayashi Takayuki Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut Yasuda Hideyo Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut Ohba Yoshiki Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut The Journal of Biochemistry Oxford University Press 119/5(1996-05), 934-939 0021-924X 119:5<934 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021332 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021332 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsukawa Torn Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut NATIONALLICENCE 700 1- Kitagawa Masatoshi Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut NATIONALLICENCE 700 1- Katayama Kazuhiko Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut NATIONALLICENCE 700 1- Nagai Yukiko Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut NATIONALLICENCE 700 1- Hayashi Takayuki Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut NATIONALLICENCE 700 1- Yasuda Hideyo Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut NATIONALLICENCE 700 1- Ohba Yoshiki Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/5(1996-05), 934-939 0021-924X 119:5<934 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford