caa a22 4500 397557817 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021327 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021327 Isolation and Characterization of the Human Inter- α-Trypsin Inhibitor Family Heavy Chain-Related Protein (IHRP) Gene (ITIHL1) [Elektronische Daten] [Ken-ichi Saguchi, Takashi Tobe, Ken Hashimoto, Yuta Nagasaki, Eiichi Oda, Yasuko Nakano, Nam-Ho Miura, Motowo Tomita] Inter-α-trypsin inhibitor (ITI) family heavy chain-related protein (IHRP) is a novel human glycoprotein that shows significant homology in amino acid sequence to proteins of the ITI family heavy chains from human plasma. Three overlapping clones that encode the human inter-c-trypsin inhibitor family heavy chain-related protein (IHRP) gene (ITIHL1) were isolated and characterized. The IHRP gene spans 15 kb and is composed of 24 exons from 27 to 207 bp in size with consensus splice sites. The gene codes for the precursor of IHRP, which is similar to inter-a-trypsin inhibitor (ITI) family heavy chains. Two major transcription initiation sites were identified in the 5′-flanking region. They contain putative promoter elements, but no typical TATA box. Some exons of this gene showed significant similarities to those of the ITI-H1 gene in nucleotide length and in intron phasing. The tissue-specific transcription of this gene may be due to the presence of binding sites for the hepatocyte nuclear factors LF-A1, HNF-5, NF-IL6, and C/EBP. This gene was found to be localized very close to another unknown gene related to EST (GenBank accession #: R54643, R50663, R50563, H27139, and R54913). © by the Journal of Biochemistry Regular Papers nationallicence IHRP gene nationallicence TATA-less promoter nationallicence Saguchi Ken-ichi Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Tobe Takashi Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Hashimoto Ken Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Nagasaki Yuta Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Oda Eiichi Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Nakano Yasuko Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Miura Nam-Ho Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Tomita Motowo Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut The Journal of Biochemistry Oxford University Press 119/5(1996-05), 898-905 0021-924X 119:5<898 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021327 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021327 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saguchi Ken-ichi Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Tobe Takashi Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Hashimoto Ken Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Nagasaki Yuta Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Oda Eiichi Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Nakano Yasuko Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Miura Nam-Ho Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Tomita Motowo Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/5(1996-05), 898-905 0021-924X 119:5<898 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford