caa a22 4500 397557825 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021316 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021316 Conversion of the Coenzyme Specificity of Isocitrate Dehydrogenase by Module Replacement [Elektronische Daten] [Takuro Yaoi, Kentaro Miyazaki, Tairo Oshima, Yutaka Komukai, Mitiko Go] The coenzyme specificity of isocitrate dehydrogenase from an extreme thennophilic bacterium was converted from NADP-dependent to NAD-dependent by replacing a "module” involved in the coenzyme binding site. The conversion was not possible with the replacement of a few residues that interact with the coenzyme. In addition, the modulereplaced mutant dehydrogenase was as stable as the original, wild type enzyme. The results support a previous hypothesis that a module is a structural and functional unit of a protein. © by the Journal of Biochemistry Rapid Communications nationallicence coenzyme specificity nationallicence exon-shuffling hypothesis nationallicence module nationallicence molecular recognition nationallicence protein engineering nationallicence Yaoi Takuro Department of Life Science, Tokyo Institute of Technology Nagatsuta, Yokohama 226 aut Miyazaki Kentaro Department of Life Science, Tokyo Institute of Technology Nagatsuta, Yokohama 226 aut Oshima Tairo Department of Life Science, Tokyo Institute of Technology Nagatsuta, Yokohama 226 aut Komukai Yutaka Department of Biology, Faculty of Science, Nagoya University Chikusa-ku, Nagoya 464-01 aut Go Mitiko Department of Biology, Faculty of Science, Nagoya University Chikusa-ku, Nagoya 464-01 aut The Journal of Biochemistry Oxford University Press 119/5(1996-05), 1014-1018 0021-924X 119:5<1014 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021316 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021316 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yaoi Takuro Department of Life Science, Tokyo Institute of Technology Nagatsuta, Yokohama 226 aut NATIONALLICENCE 700 1- Miyazaki Kentaro Department of Life Science, Tokyo Institute of Technology Nagatsuta, Yokohama 226 aut NATIONALLICENCE 700 1- Oshima Tairo Department of Life Science, Tokyo Institute of Technology Nagatsuta, Yokohama 226 aut NATIONALLICENCE 700 1- Komukai Yutaka Department of Biology, Faculty of Science, Nagoya University Chikusa-ku, Nagoya 464-01 aut NATIONALLICENCE 700 1- Go Mitiko Department of Biology, Faculty of Science, Nagoya University Chikusa-ku, Nagoya 464-01 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/5(1996-05), 1014-1018 0021-924X 119:5<1014 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford