caa a22 4500 397557841 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021330 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021330 Effect of Macromolecular-Translocation Inhibitor-Ill on Binding of Activated Glucocorticoid-Receptor Complex to Specific DNA [Elektronische Daten] [Kazuki Okamoto, Gang Liu, Wen-Gong Yu, Tetsuji Ochiai, Fumihide Isohashi] We previously purified macromolecular-translocation inhibitor-Ill (MTI-III), which inhibits the binding of the activated glucocorticoid-receptor complex (GR) to nuclei, to homogeneity from rat liver, and we found that the purified MTI-III bound to partially purified activated GR under low salt conditions at slightly acidic pH [Liu, G., Okamoto, K., and Isohashi, F. (1993) Eur. J. Biochem;. 218, 679-687]. This was the first direct evidence that the inhibitor acts through a direct interaction with the activated GR. In this study, we examined whether the purified MTI-III could interfere with the binding of GR to a DNA fragment containing a specific glucocorticoid-response element (GRE). Under nearly isotonic salt conditions at neutral pH, the activated GR bound to the GRE but not to nonspecific DNA. Under similar conditions, the activated GR also bound to the purified MTI-III. The resulting GR/MTI-III complex did not bind to the GRE. We also found that addition of MTI-III to the GR/GRE complex resulted in time-dependent disruption of the GR/GRE complex and formation of the GR/MTI-III complex. The half-life of the GR/GRE complex in the presence of MTI-III was about 13 min. These results suggest that MTI-III enhances the release of GR from the GR/GRE complex and immediately forms a stable GR/MTI-III complex. © by the Journal of Biochemistry Regular Papers nationallicence glucocorticoid-receptor nationallicence glucocorticoid-response element nationallicence translocation-in-hibitor nationallicence Okamoto Kazuki Department of Biochemistry, St Maiianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216 aut Liu Gang Department of Biochemistry, St Maiianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216 aut Yu Wen-Gong Department of Biochemistry, St Maiianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216 aut Ochiai Tetsuji Department of Biochemistry, St Maiianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216 aut Isohashi Fumihide Department of Biochemistry, St Maiianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216 aut The Journal of Biochemistry Oxford University Press 119/5(1996-05), 920-925 0021-924X 119:5<920 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021330 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021330 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okamoto Kazuki Department of Biochemistry, St Maiianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216 aut NATIONALLICENCE 700 1- Liu Gang Department of Biochemistry, St Maiianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216 aut NATIONALLICENCE 700 1- Yu Wen-Gong Department of Biochemistry, St Maiianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216 aut NATIONALLICENCE 700 1- Ochiai Tetsuji Department of Biochemistry, St Maiianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216 aut NATIONALLICENCE 700 1- Isohashi Fumihide Department of Biochemistry, St Maiianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/5(1996-05), 920-925 0021-924X 119:5<920 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford