caa a22 4500 39755785X CHVBK 20180308164808.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021329 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021329 Immunofluorescence Localization of a 23-kDa Tetrahymena; Calcium-Binding Protein, TCBP-23, in the Cell Cortex [Elektronische Daten] [Kazuko Hanyu, Osamu Numata, Mihoko Takahashi, Yoshio Watanabe] Previously, we succeeded in cloning a cDNA of the 23-kDa Tetrahymena Ca2+-binding protein, designated TCBP-23. Analysis of the deduced amino acid sequences showed that TCBP-23 is a member of the EF-hand family of Ca2+-binding proteins. However, its physiological function was not elucidated. In the studies reported here, recombinant TCBP-23 was expressed in Escherichia coli and purified. Since recombinant TCBP-23 binds Ca2+ in vitro, Ca2+-binding domains of the protein are likely to be functional in vivo. Rabbit antibodies against TCBP-23 were raised and used to determine the intracellular localization of the protein in Tetrahymena cells by indirect immunofluorescence. The antibodies strongly stained the whole cell cortex except for the oral apparatus and around the basal bodies. TCBP-23 remained in detergent-extracted cells, suggesting that it is associated with the epiplasm, the membrane skeleton of Tetrahymena. These results suggest that TCBP-23 may mediate Ca2+-regulated processes in the cell cortex. © by the Journal of Biochemistry Regular Papers nationallicence Ca+-binding protein nationallicence cell cortex nationallicence EF-hand nationallicence localization nationallicence Tetrahymena nationallicence Hanyu Kazuko Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 aut Numata Osamu Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 aut Takahashi Mihoko Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 aut Watanabe Yoshio Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 aut The Journal of Biochemistry Oxford University Press 119/5(1996-05), 914-919 0021-924X 119:5<914 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021329 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021329 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hanyu Kazuko Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Numata Osamu Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Takahashi Mihoko Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Watanabe Yoshio Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/5(1996-05), 914-919 0021-924X 119:5<914 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford