caa a22 4500 397557884 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021467 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021467 Identification of an Interleukin-1β Converting Enzyme-Like Activity That Increases upon Treatment of P19 Cells with Retinoic Acid as the Proteasome [Elektronische Daten] [Tatsuyiki Kobayashi, Ayako Shinozaki, Takashi Momoi, Kiichi Arahata, Toshifumi Tsukahara] We examined changes in proteinase activities in Pl9 embryonal carcinoma cells during retinoic acid-induced differentiation. The interleukin-1β converting enzyme (ICE)-like Ac-YVAD-MCA hydrolytic activity was increased about 6-fold by treatment with retinoic acid. This activity was inhibited by N-ethylmaleimide and Ac-YVAD-H but not by E-64, EDTA, PMSF, or amastatin. The ICE-like activity in P19 cells eluted as a single peak just after the void volume on gel filtration. No ICE-like activity was observed at a molecular mass of 30-50 kDa. Enzymatic purification, Western blot analysis, and an immunoabsorption study demonstrated that the ICE-like activity in P19 cells is caused by the proteasome, and is stimulated during retinoic acid-induced differentiation. The proteasome purified from mouse liver also cleaved Ac-YVAD-MCA. These results strongly suggest that the proteasome is a major ICE-like proteinam in P19 cells and may be involved in the neural differentiation and the apoptotic pathway. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence apoptosis nationallicence ICE-like activity nationallicence neural differentiation nationallicence P19 cells nationallicence proteasome nationallicence Kobayashi Tatsuyiki Department of Neuromuscular Research, National Institute of Neuroscience, NCNP Ogawahigashi-cho 4-1-1, Kodaira, Tokyo 187 aut Shinozaki Ayako Department of Neuromuscular Research, National Institute of Neuroscience, NCNP Ogawahigashi-cho 4-1-1, Kodaira, Tokyo 187 aut Momoi Takashi Department of Development and Differentiation, National Institute of Neuroscience, NCNP Ogawahigashi-cho 4-1-1, Kodaira, Tokyo 187 aut Arahata Kiichi Department of Neuromuscular Research, National Institute of Neuroscience, NCNP Ogawahigashi-cho 4-1-1, Kodaira, Tokyo 187 aut Tsukahara Toshifumi Department of Neuromuscular Research, National Institute of Neuroscience, NCNP Ogawahigashi-cho 4-1-1, Kodaira, Tokyo 187 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 699-704 0021-924X 120:4<699 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021467 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021467 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kobayashi Tatsuyiki Department of Neuromuscular Research, National Institute of Neuroscience, NCNP Ogawahigashi-cho 4-1-1, Kodaira, Tokyo 187 aut NATIONALLICENCE 700 1- Shinozaki Ayako Department of Neuromuscular Research, National Institute of Neuroscience, NCNP Ogawahigashi-cho 4-1-1, Kodaira, Tokyo 187 aut NATIONALLICENCE 700 1- Momoi Takashi Department of Development and Differentiation, National Institute of Neuroscience, NCNP Ogawahigashi-cho 4-1-1, Kodaira, Tokyo 187 aut NATIONALLICENCE 700 1- Arahata Kiichi Department of Neuromuscular Research, National Institute of Neuroscience, NCNP Ogawahigashi-cho 4-1-1, Kodaira, Tokyo 187 aut NATIONALLICENCE 700 1- Tsukahara Toshifumi Department of Neuromuscular Research, National Institute of Neuroscience, NCNP Ogawahigashi-cho 4-1-1, Kodaira, Tokyo 187 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 699-704 0021-924X 120:4<699 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford