caa a22 4500 397557892 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021483 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021483 Isolation and Characterization of GBP28, a Novel Gelatin-Binding Protein Purified from Human Plasma [Elektronische Daten] [Yasuko Nakano, Takashi Tobe, Nam-Ho Choi-Miura, Toshio Mazda, Motowo Tomita] By use of its affinity to gelatin-Cellulofine, a novel protein, GBP28 (gelatin-binding protein of 28 kDa), was obtained from human plasma. GBP28 bound to gelatin-Cellulofine could be eluted with 1 M NaCl. By analysis of its amino-terminal amino acid sequences and the peptides obtained by protease digestion, GBP28 was identified as a novel protein. After repeated gel chromatography of the 1 M NaCl eluate from gelatin-Cellulofine, about 50 μg of GBP28 was purified from 500 ml of human plasma. On gel chromatography, the protein migrated as a molecule of about 420 kDa. On SDS-PAGE, its molecular mass was 28 kDa under reducing conditions and 68 kDa under nonreducing conditions. Recently, human mRNA specific to adipose tissue, cDNA clone apM1, has been registered [Maeda, K., Okubo, K., Shimomura, I., Funahashi, T., Matsuzawa, Y., and Matsubara, K. (1996) Biochem. Biophys. Res. Commun. 221, 286-289]. The assumed amino acid sequence of cDNA clone apM1 contained all the sequences of GBP28 and its peptides. Therefore, it is evident that the cDNA clone apM1 encodes GBP28 and the protein is specific to adipose tissue. The clone encodes a polypeptide of 244 amino acids with a secretory signal sequence at the amino terminus, a small non-helical region, a stretch of 22 collagen repeats and a globular domain. Thus, GBP28 appears to belong to a family of proteins possessing a collagen-like domain through which they form homo-trimers, which further combine to make oligomeric complexes. Although its biological function is presently unclear, its adipocyte-specific expression suggests that GBP28 may function as an endogenous factor involved in lipid catabolism and storage or whole body metabolism. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence adipocyte nationallicence collagen-like domain nationallicence extracellular matrix nationallicence GBP28 nationallicence gelatin-binding nationallicence Nakano Yasuko Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Tobe Takashi Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Choi-Miura Nam-Ho Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Mazda Toshio Japanese Red Cross Tokyo Blood Center Sakaiminami-cho, Musashino, Tokyo 180 aut Tomita Motowo Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 803-812 0021-924X 120:4<803 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021483 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021483 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakano Yasuko Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Tobe Takashi Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Choi-Miura Nam-Ho Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Mazda Toshio Japanese Red Cross Tokyo Blood Center Sakaiminami-cho, Musashino, Tokyo 180 aut NATIONALLICENCE 700 1- Tomita Motowo Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 803-812 0021-924X 120:4<803 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford