caa a22 4500 397557906 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021468 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021468 Sphingosine Inhibition of NADPH Oxidase Activation in a Cell-Free System [Elektronische Daten] [Jun-ichi Sasaki, Masafumi Yamaguchi, Satoshi Saeki, Hana Yamane, Naoki Okamura, Sadahiko Ishibashi] The effects of sphingoid bases, sphingosine and dihydrosphingosine, which are protein kinase C (PKC) inhibitors, on NADPH oxidase were examined in a cell-free system. The bases inhibited cell-free activation of NADPH oxidase by arachidonic acid at lower concentration than N-acetylsphingosine. Thus, positive charge In the molecules may play a critical role in inhibition of the oxidase. Sphingosine did not change the Km, value for NADPH, but shifted the optimum concentration of arachidonic acid for activation of the oxidase. Moreover, sphingosine suppressed the translocation of p47-phox, one of the cytosolic components of the oxidase, to the membrane fraction, suggesting that the base inhibits the assembly of the components. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence NADPH oxidase nationallicence neutrophil nationallicence sphingolipids nationallicence sphingosine nationallicence superoxide anion nationallicence Sasaki Jun-ichi Department of Physiological Chemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut Yamaguchi Masafumi Department of Physiological Chemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut Saeki Satoshi Department of Physiological Chemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut Yamane Hana Department of Physiological Chemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut Okamura Naoki Department of Physiological Chemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut Ishibashi Sadahiko Department of Physiological Chemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 705-709 0021-924X 120:4<705 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021468 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021468 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sasaki Jun-ichi Department of Physiological Chemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut NATIONALLICENCE 700 1- Yamaguchi Masafumi Department of Physiological Chemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut NATIONALLICENCE 700 1- Saeki Satoshi Department of Physiological Chemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut NATIONALLICENCE 700 1- Yamane Hana Department of Physiological Chemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut NATIONALLICENCE 700 1- Okamura Naoki Department of Physiological Chemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut NATIONALLICENCE 700 1- Ishibashi Sadahiko Department of Physiological Chemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 705-709 0021-924X 120:4<705 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford