caa a22 4500 397557914 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021471 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021471 Identification of a Novel Growth-Promoting Factor with a Wide Target Cell Spectrum from Various Tumor Cells as Catalase [Elektronische Daten] [Tomomi Miyamoto, Masaru Hayashi, Akihiko Takeuchi, Takayuki Okamoto, Sachiko kawashima, Takemasa Takii, Hidetoshi Hayashi, Kikuo Onozaki] We have previously reported the purification from human erythrocyte extracts of a novel growth-promoting factor with a wide target cell spectrum. The factor has been identified as catalase. As cell extracts from a variety of tumor cell types exhibited both growth-promoting and catalase activities, the relationship between the two activities was examined using cell extracts from three different cell types, human myeloid cells (U937), human melanoma cells (A375-C6), and human B cells (Daudi). The growth-promoting and catalase activities were well correlated in these cell extracts. The antibody against human catalase absorbed not only catalase activity, but also the growth-promoting activity of extracts from these cell types. Treatment of the cell extracts from these cells with an irreversible catalase inhibitor, aminotriazole, abolished both the catalase and growth-promoting activities. In contrast, glutathione peroxidase (GSH-Px) activity was neither absorbed with the anti-catalase antibody, nor inhibited by aminotriazole. In addition, GSH-Px exhibited growth-promoting activity only in the presence of glutathione (GSH). These results, in conjunction with the effect of aminotriazole on the growth-promoting activity of catalase, suggest that catalase is the major growth-promoting molecule in the cell extractsand H2O2-decomposing activity is important. Northern blot analysis revealed that these cells contained authentic catalase mRNA, and the mRNA level was compatible with the catalase and growth-promoting activities in the cell extracts. These results suggest that the growth-promoting activity in the tumor cell extracts is due to catalase. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence catalese nationallicence growth factor nationallicence H2O2 nationallicence reactive oxygens nationallicence tumor cells nationallicence Miyamoto Tomomi Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut Hayashi Masaru Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut Takeuchi Akihiko Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut Okamoto Takayuki Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut kawashima Sachiko Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut Takii Takemasa Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut Hayashi Hidetoshi Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut Onozaki Kikuo Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 725-730 0021-924X 120:4<725 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021471 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021471 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miyamoto Tomomi Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut NATIONALLICENCE 700 1- Hayashi Masaru Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut NATIONALLICENCE 700 1- Takeuchi Akihiko Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut NATIONALLICENCE 700 1- Okamoto Takayuki Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut NATIONALLICENCE 700 1- kawashima Sachiko Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut NATIONALLICENCE 700 1- Takii Takemasa Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut NATIONALLICENCE 700 1- Hayashi Hidetoshi Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut NATIONALLICENCE 700 1- Onozaki Kikuo Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 725-730 0021-924X 120:4<725 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford