caa a22 4500 397557922 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021469 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021469 Steroid Hormones Differentially Induce Transcription of the Chicken Ovalbumin Gene, but Stabilize the mRNA with the Same Half-Life [Elektronische Daten] [Yukitomo Arao, Naoto Miyatake, Etsuko Yamamoto, Hiroaki Usiku, Akiko Matsuura, Yuichi Ninomiya, Shoichi Masushige, Tadao Hasegawa, Shigeaki Kato] The stabilization of chicken ovalbumin (OVA) mRNA by different classes of steroid hormones (estrogen, progesterone, glucocorticoid, and androgen) was studied in the oviducts of chicks treated with combinations of four steroids. The combination of estrogen with progesterone, glucocorticoid, or androgen enhanced the induction of the OVA gene more than did estrogen alone. Run-on analysis of the isolated oviduct nuclei to measure the transcription rate of the OVA gene showed that the enhanced induction of the OVA gene by the combined hormone treatments was partly caused by an increased rate of transcription. The half-life of OVA mRNA as determined using a transcription inhibitor (actinomycin D) was estimated to be about 24 h irrespective of the hormone treatment, though the half-life was about 6 h in the absence of hormones. These results suggested that the prolongation of the half-life of OVA mRNA by steroid hormones is constant irrespective of differential transcription rates of the OVA gene. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence mRNA stability nationallicence nuclear receptor nationallicence post-transcription regulation nationallicence sterioid hormones nationallicence Arao Yukitomo Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut Miyatake Naoto Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut Yamamoto Etsuko Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut Usiku Hiroaki Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut Matsuura Akiko Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut Ninomiya Yuichi Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut Masushige Shoichi Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut Hasegawa Tadao Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut Kato Shigeaki Institute of Molecular and Cellular Biosciences, The University of Tokyo Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 710-715 0021-924X 120:4<710 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021469 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021469 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arao Yukitomo Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut NATIONALLICENCE 700 1- Miyatake Naoto Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut NATIONALLICENCE 700 1- Yamamoto Etsuko Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut NATIONALLICENCE 700 1- Usiku Hiroaki Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut NATIONALLICENCE 700 1- Matsuura Akiko Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut NATIONALLICENCE 700 1- Ninomiya Yuichi Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut NATIONALLICENCE 700 1- Masushige Shoichi Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut NATIONALLICENCE 700 1- Hasegawa Tadao Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Sakuragaoka Setagaya-ku, Tokyo 156 aut NATIONALLICENCE 700 1- Kato Shigeaki Institute of Molecular and Cellular Biosciences, The University of Tokyo Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 710-715 0021-924X 120:4<710 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford