caa a22 4500 397557930 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021480 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021480 Lower Activation Energy for Sliding of F-Actin on a Less Thermostable Isoform of Carp Myosin [Elektronische Daten] [Shigeru Chaen, Misako Nakaya, Xiao-Feng Guo, Shugo Watabe] We have examined the temperature-dependence of sliding velocity of fluorescent F-actin on myosins isolated from 10°C- and 30°C-acclimated carp. Activation energies for the sliding of F-actin were 63 and 111 kJ/mol for the 10°C- and 30°C-acclimated carp myosins, respectively. Arrhenius plots of the sliding velocity from 10°C- and 30°C-acclimated carp myosin were shown to intersect at high temperature (about 30°C). The thermostability estimated by measuring the Ca2+-ATPase activity was less for myosin from 10°C- than 30°C-acclimated carp. We suggest that a less thermostable structure in cold-acclimated carp myosin results in a reduced activation energy for the contractile process, which allows the F-actin to slide fast even at low temperatures. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence activation energy nationallicence carp myosin nationallicence in vitro motility assay nationallicence temperature acclimation nationallicence thermostability nationallicence Chaen Shigeru Department of Physiology, School of Medicine, Teikyo University Itabashi-ku, Tokyo 173 aut Nakaya Misako Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo Bukyo-ku, Tokyo 113 aut Guo Xiao-Feng Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo Bukyo-ku, Tokyo 113 aut Watabe Shugo Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo Bukyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 788-791 0021-924X 120:4<788 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021480 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021480 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chaen Shigeru Department of Physiology, School of Medicine, Teikyo University Itabashi-ku, Tokyo 173 aut NATIONALLICENCE 700 1- Nakaya Misako Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo Bukyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Guo Xiao-Feng Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo Bukyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Watabe Shugo Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo Bukyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 788-791 0021-924X 120:4<788 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford