caa a22 4500 397557973 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021484 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021484 Structure and Intracellular Localization of Mouse ADP-Ribosylation Factors Type 1 to Type 6 (ARF1-ARF6) [Elektronische Daten] [Masahiro Hosaka, Kyoko Toda, Hiroyuki Takatsu, Seiji Torii, Kazuo Murakami, Kazuhisa Nakayama] ADP-ribosylation factors (ARFs) are a family of small GTP-binding proteins that are proposed to be involved in the formation of coated transport vesicles. Although six ARF sequences have been reported in mammals to date, it has been unclear how many ARF members are present in a single organism. In this study, we provide the first direct evidence by cDNA cloning for the presence of all six ARF members in mouse. These proteins are highly conserved across mammalian species and Northern blot analysis revealed that mRNAs for all the members were expressed ubiquitously. Transfection of cells with epitope-tagged ARFs revealed that ARFs 1-3 displayed a perinuclear Golgi localization, while ARFs 4-6 appeared to be widely dispersed throughout the cytoplasm. These results suggest that although all the ARF proteins play fundamental and critical roles in cellular function, they are involved in different vesicular transport processes. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence ADP-ribosylation factor nationallicence epitope tagging nationallicence Golgi complex nationallicence small GTP-binding protein nationallicence vesicular trafficking nationallicence Hosaka Masahiro Institute of Biological Sciences Tsukuba, Ibaraki 305 aut Toda Kyoko Institute of Applied Biochemistry, University of Tsukuba Tsukuba, Ibaraki 305 aut Takatsu Hiroyuki Institute of Biological Sciences Tsukuba, Ibaraki 305 aut Torii Seiji Institute of Applied Biochemistry, University of Tsukuba Tsukuba, Ibaraki 305 aut Murakami Kazuo Institute of Applied Biochemistry, University of Tsukuba Tsukuba, Ibaraki 305 aut Nakayama Kazuhisa Institute of Biological Sciences Tsukuba, Ibaraki 305 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 813-819 0021-924X 120:4<813 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021484 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021484 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hosaka Masahiro Institute of Biological Sciences Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Toda Kyoko Institute of Applied Biochemistry, University of Tsukuba Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Takatsu Hiroyuki Institute of Biological Sciences Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Torii Seiji Institute of Applied Biochemistry, University of Tsukuba Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Murakami Kazuo Institute of Applied Biochemistry, University of Tsukuba Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Nakayama Kazuhisa Institute of Biological Sciences Tsukuba, Ibaraki 305 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 813-819 0021-924X 120:4<813 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford