caa a22 4500 397558015 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021478 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021478 Molecular Cloning of a Novel Protein Containing the Association Domain of Calmodulin-Dependent Protein Kinase II [Elektronische Daten] [Rika Sugai, Masayuki Takeuchi, Sachiko Okuno, Hitoshi Fujisawa] The cDNA of a novel protein, which contains the association domain of α isoform of calmodulin-dependent protein kinase II (CaM-kinase IIα), was cloned from rat skeletal muscle. This protein, called αKAP, consisted of 200 amino acid residues with a molecular weight of 22,583. αKAP has a highly hydrophobic amino-terminal stretch of 25 amino acids which is absent from CaM-kinase IIα, suggesting that this protein is either a secretory protein or an integral membrane protein. Northern blot analysis with a probe specific for αKAP detected three distinct mRNA species of 4.0, 2.4, and 1.5 kb in rat skeletal muscle. The 4.0- and 2.4-kb RNAs were also detected in heart, and at much lower levels in lung, kidney, and testis. Western blot analysis, using antibody raised against a synthetic peptide corresponding to the carboxyl-terminal 15 amino acids, revealed a single band corresponding in mobility to a molecular weight of 21,000 In crude extracts of both rat skeletal muscle and bacteria transformed with the cDNA, suggesting that no significant post-translational modification, such as excision of the amino-terminal hydrophobic segment, occurred. This, together with the fact that αKAP was recovered In the high-speed pellet in skeletal muscle, indicated that this protein may be an integral membrane protein. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence Cam-kinase II nationallicence cDNA sequence nationallicence αKAP nationallicence signal sequence nationallicence skeletal muscle nationallicence Sugai Rika Department of Biochemistry, Asahikawa Medical College, Asahikawo Hokkaido 078 aut Takeuchi Masayuki Department of Biochemistry, Asahikawa Medical College, Asahikawo Hokkaido 078 aut Okuno Sachiko Department of Biochemistry, Asahikawa Medical College, Asahikawo Hokkaido 078 aut Fujisawa Hitoshi Department of Biochemistry, Asahikawa Medical College, Asahikawo Hokkaido 078 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 773-779 0021-924X 120:4<773 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021478 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021478 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sugai Rika Department of Biochemistry, Asahikawa Medical College, Asahikawo Hokkaido 078 aut NATIONALLICENCE 700 1- Takeuchi Masayuki Department of Biochemistry, Asahikawa Medical College, Asahikawo Hokkaido 078 aut NATIONALLICENCE 700 1- Okuno Sachiko Department of Biochemistry, Asahikawa Medical College, Asahikawo Hokkaido 078 aut NATIONALLICENCE 700 1- Fujisawa Hitoshi Department of Biochemistry, Asahikawa Medical College, Asahikawo Hokkaido 078 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 773-779 0021-924X 120:4<773 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford