caa a22 4500 39755804X CHVBK 20180308164808.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021472 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021472 Individual Expression of Candida tropicalis Peroxisomal and Mitochondrial Carnitine Acetyltransferase-Encoding Genes and Subcellular Localization of the Products in Saccharomyces cerevisiae [Elektronische Daten] [Hiroyuki Kawachi, Haruyuki Atomi, Mitsuyoshi Ueda, Noriko Hashimoto, Keiko Kobayashi, Tomoko Yoshida, Naomi Kamasawa, Masako Osumi, Atsuo Tanaka] In an n-alkane-assimilating yeast, Candida tropicalis, carnitine acetyltransferase (CAT; EC 2.3.1.7) was localized in both peroxisomes and mitochondria. Both CATs were encoded by one gene, CT-CAT, although the initiation sites of translation were suggested to be different. In the present study, the genes corresponding to the supposed C. tropicalis peroxisomal and mitochondrial CATs, which were truncated from the CT-CAT gene, were individually expressed in Saccharomyces cerevisiae, using the C. tropicalis isocitrate lyase promoter (UPR-ICL), which is inducible by oleic acid in concert with proliferation of peroxisomes in S. cerevisiae [Umemura, K., Atomi, H., Kanai, T., Teranishi, Y., Ueda, M., and Tanaka, A. (1995) Appl. Microbiol. Biotechnol. 43, 489-492]. The 71 kDa precursor of mitochondrial CAT, initiating at the first Met, was found to be processed to the mature size (66 kDa) in S. cerevisiae and immunoelectronmicroscopical observation revealed that this enzyme was localized in mitochondria. On the other hand, 68 kDa CAT, initiating at the second Met (residue No. 19), had no cleavable signal and was translocated into peroxisomes and cytosol, but not into mitochondria. The aniino-terniinal amino acid sequences of individually expressed CATs were identical to those of CATs isolated from alkane-grown C. tropicalls cells, respectively. These results demonstrated that only the 71 kDa protein yielded the 66 kDa protein and that peroxisomal and mitochondrial CATs arose from the difference in the initiation sites of translation. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence Candida tropicalis nationallicence carnitine acetyltransferase nationallicence initiation of traslation nationallicence mitochondria nationallicence peroxisomes nationallicence Kawachi Hiroyuki Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University Yoshida, Sakyo-ku, Kyoto 606-01 aut Atomi Haruyuki Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University Yoshida, Sakyo-ku, Kyoto 606-01 aut Ueda Mitsuyoshi Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University Yoshida, Sakyo-ku, Kyoto 606-01 aut Hashimoto Noriko Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University Mejiro-dai, Bunkyo-ku, Tokyo 112 aut Kobayashi Keiko Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University Mejiro-dai, Bunkyo-ku, Tokyo 112 aut Yoshida Tomoko Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University Mejiro-dai, Bunkyo-ku, Tokyo 112 aut Kamasawa Naomi Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University Mejiro-dai, Bunkyo-ku, Tokyo 112 aut Osumi Masako Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University Mejiro-dai, Bunkyo-ku, Tokyo 112 aut Tanaka Atsuo Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University Yoshida, Sakyo-ku, Kyoto 606-01 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 731-735 0021-924X 120:4<731 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021472 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021472 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kawachi Hiroyuki Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University Yoshida, Sakyo-ku, Kyoto 606-01 aut NATIONALLICENCE 700 1- Atomi Haruyuki Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University Yoshida, Sakyo-ku, Kyoto 606-01 aut NATIONALLICENCE 700 1- Ueda Mitsuyoshi Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University Yoshida, Sakyo-ku, Kyoto 606-01 aut NATIONALLICENCE 700 1- Hashimoto Noriko Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University Mejiro-dai, Bunkyo-ku, Tokyo 112 aut NATIONALLICENCE 700 1- Kobayashi Keiko Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University Mejiro-dai, Bunkyo-ku, Tokyo 112 aut NATIONALLICENCE 700 1- Yoshida Tomoko Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University Mejiro-dai, Bunkyo-ku, Tokyo 112 aut NATIONALLICENCE 700 1- Kamasawa Naomi Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University Mejiro-dai, Bunkyo-ku, Tokyo 112 aut NATIONALLICENCE 700 1- Osumi Masako Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University Mejiro-dai, Bunkyo-ku, Tokyo 112 aut NATIONALLICENCE 700 1- Tanaka Atsuo Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University Yoshida, Sakyo-ku, Kyoto 606-01 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 731-735 0021-924X 120:4<731 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford