caa a22 4500 397558058 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021481 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021481 Purification and Characterization of Arginine:Mono-ADP-Ribosylhydrolase from Euglena gracilis Z [Elektronische Daten] [Shigeo Takenaka, Masuda Wataru, Shingo Tsuyama, Yoshiyuki Tamura, Kazutaka Miyatake, Yoshihisa Nakano] Arginine:mono-ADP-ribosylhydrolase was purified from a protozoan, Euglena gracilis Z, using [32P]mono-ADP-ribosylated actin as a substrate. The enzyme showed molecular mass of 33 kDa both in SDS PAGE and gel filtration, indicating it to be a monomeric protein. It was strongly inhibited by ADP and ADP-ribose and activated by Mg2+, DTT, and 2-mercaptoethanol. These results suggest that it recognizes the ADP-ribose moiety of the modified protein. Since the enzyme activity increased in S phase and late G0 phase in a synchronous dividing culture, the enzyme may function in the regulation of the cell cycle. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence ADP-ribosylhydrolase nationallicence cell cycle nationallicence Euglena gracilis nationallicence mono-ADP-ribosylation nationallicence NAD+ nationallicence Takenaka Shigeo Department of Applied Biological Chemistry, Osaka Prefecture University Sakai, Osaka 593 aut Wataru Masuda Department of Applied Biological Chemistry, Osaka Prefecture University Sakai, Osaka 593 aut Tsuyama Shingo Department of Veterinary Science, Osaka Prefecture University Sakai, Osaka 593 aut Tamura Yoshiyuki Laboratory of Nutrition and Food Science, Hagoromo Gakuen Junior College Sakai, Osaka 592 aut Miyatake Kazutaka Department of Applied Biological Chemistry, Osaka Prefecture University Sakai, Osaka 593 aut Nakano Yoshihisa Department of Applied Biological Chemistry, Osaka Prefecture University Sakai, Osaka 593 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 792-796 0021-924X 120:4<792 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021481 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021481 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takenaka Shigeo Department of Applied Biological Chemistry, Osaka Prefecture University Sakai, Osaka 593 aut NATIONALLICENCE 700 1- Wataru Masuda Department of Applied Biological Chemistry, Osaka Prefecture University Sakai, Osaka 593 aut NATIONALLICENCE 700 1- Tsuyama Shingo Department of Veterinary Science, Osaka Prefecture University Sakai, Osaka 593 aut NATIONALLICENCE 700 1- Tamura Yoshiyuki Laboratory of Nutrition and Food Science, Hagoromo Gakuen Junior College Sakai, Osaka 592 aut NATIONALLICENCE 700 1- Miyatake Kazutaka Department of Applied Biological Chemistry, Osaka Prefecture University Sakai, Osaka 593 aut NATIONALLICENCE 700 1- Nakano Yoshihisa Department of Applied Biological Chemistry, Osaka Prefecture University Sakai, Osaka 593 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 792-796 0021-924X 120:4<792 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford