caa a22 4500 397558082 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021488 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021488 Cloning, Expression and Characterization of Plasma Platelet-Activating Factor-Acetylhydrolase from Guinea Pig [Elektronische Daten] [Ken Karasawa, Osamu Kuge, Kiyoshi Kawasaki, Masahiro Nishijima, Yasuko Nakano, Motowo Tomita, Kazuaki Yokoyama, Morio Setaka, Shoshichi Nojima] In a previous study, we purified PAF-acetylhydrolase, which converts PAF to an inactive metabolite, lysoPAF, from peritoneal fluid of guinea pigs subjected to experimental endotoxin shock and found that this purified enzyme had similar biochemical properties to the plasma enzyme [Karasawa, K., Yato, M., Setaka, M., and Nojima, S. (1994) J. Biochem. 116,374-379]. In this study, we isolated a homogeneous enzyme preparation from guinea pig plasma using a similar procedure. The molecular mass of this purified enzyme, as determined by SDS-PAGE was 58-63 kDa, larger than that (43 kDa) of the human enzyme. To elucidate the molecular structure of this enzyme and clarify its relationships with PAF-acetylhydrolasea of other species, we isolated and sequenced a cDNA encoding this enzyme. Its cDNA contains an open reading frame encoding 436 amino acids and its predicted molecular mass (49 kDa) is lower than that of the native enzyme, suggesting that guinea pig plasma PAF-acetylhydrolase, unlike the human enzyme, is modified post-translationally, perhaps by glycosylation. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence cDNA cloning nationallicence guinea pig nationallicence PAF nationallicence PAF-acetylhydrolase nationallicence purification nationallicence Karasawa Ken Faculty of Pharmaceutical Sciences, Teikyo University Sagamiko, Kanagawa 199-01 aut Kuge Osamu Department of Cell Biology, National Institute of Health of Japan Shinjuku-ku, Tokyo aut Kawasaki Kiyoshi Department of Cell Biology, National Institute of Health of Japan Shinjuku-ku, Tokyo aut Nishijima Masahiro Department of Cell Biology, National Institute of Health of Japan Shinjuku-ku, Tokyo aut Nakano Yasuko Faculty of Pharmaceutical Sciences, Showa University Shinagawa-ku, Tokyo 142 aut Tomita Motowo Faculty of Pharmaceutical Sciences, Showa University Shinagawa-ku, Tokyo 142 aut Yokoyama Kazuaki Faculty of Pharmaceutical Sciences, Teikyo University Sagamiko, Kanagawa 199-01 aut Setaka Morio Faculty of Pharmaceutical Sciences, Teikyo University Sagamiko, Kanagawa 199-01 aut Nojima Shoshichi Faculty of Pharmaceutical Sciences, Teikyo University Sagamiko, Kanagawa 199-01 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 838-844 0021-924X 120:4<838 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021488 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021488 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Karasawa Ken Faculty of Pharmaceutical Sciences, Teikyo University Sagamiko, Kanagawa 199-01 aut NATIONALLICENCE 700 1- Kuge Osamu Department of Cell Biology, National Institute of Health of Japan Shinjuku-ku, Tokyo aut NATIONALLICENCE 700 1- Kawasaki Kiyoshi Department of Cell Biology, National Institute of Health of Japan Shinjuku-ku, Tokyo aut NATIONALLICENCE 700 1- Nishijima Masahiro Department of Cell Biology, National Institute of Health of Japan Shinjuku-ku, Tokyo aut NATIONALLICENCE 700 1- Nakano Yasuko Faculty of Pharmaceutical Sciences, Showa University Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Tomita Motowo Faculty of Pharmaceutical Sciences, Showa University Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Yokoyama Kazuaki Faculty of Pharmaceutical Sciences, Teikyo University Sagamiko, Kanagawa 199-01 aut NATIONALLICENCE 700 1- Setaka Morio Faculty of Pharmaceutical Sciences, Teikyo University Sagamiko, Kanagawa 199-01 aut NATIONALLICENCE 700 1- Nojima Shoshichi Faculty of Pharmaceutical Sciences, Teikyo University Sagamiko, Kanagawa 199-01 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 838-844 0021-924X 120:4<838 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford