caa a22 4500 397558090 CHVBK 20180308164808.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021474 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021474 Characterization of Ficolins as Novel Elastin-Binding Proteins and Molecular Cloning of Human Ficolin-1 [Elektronische Daten] [Satoru Harumiya, Kohsuke Takeda, Tsukasa Sugiura, Yuki Fukumoto, Hiroyuki Tachikawa, Kohei Miyazono, Daisaburo Fujimoto, Hidenori Ichijo] A novel elastin-binding protein, EBP-37, was recently identified and purified from human plasma. Its partial amino acid sequences showed significant homology to porcine ficolins, which were originally purified from porcine uterus membranes as multimeric proteins with fibrinogen-and collagen-like domains. Here we report the presence of flcolins in an elastin-binding fraction of porcine plasma and the direct binding of recombinant porcine ficolin-α to elastin. In addition, a cDNA encoding a human counterpart of porcine ficolins that is composed of 319 amino acids and is different from EBP-37 was cloned and named human flcolin-1. Northern blotting of various human tissues revealed that human ficolln-1 mRNA is highly expressed in peripheral blood leukocytes. These data suggested that there are at least two kinds of ficolin-related proteins in both pig and human, and they may function as plasma proteins with elastin-binding activities. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence elastin nationallicence elastin-binding protein nationallicence ficolin nationallicence plasma nationallicence transforming growth factor-β1 nationallicence Harumiya Satoru Department of Biochemistry, The Cancer Institute, Tokyo, Japanese Foundation for Cancer Research 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170 aut Takeda Kohsuke Department of Biochemistry, The Cancer Institute, Tokyo, Japanese Foundation for Cancer Research 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170 aut Sugiura Tsukasa Laboratory of Biological Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo 183 aut Fukumoto Yuki Laboratory of Biological Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo 183 aut Tachikawa Hiroyuki Laboratory of Biological Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo 183 aut Miyazono Kohei Department of Biochemistry, The Cancer Institute, Tokyo, Japanese Foundation for Cancer Research 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170 aut Fujimoto Daisaburo Laboratory of Biological Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo 183 aut Ichijo Hidenori Department of Biochemistry, The Cancer Institute, Tokyo, Japanese Foundation for Cancer Research 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 745-751 0021-924X 120:4<745 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021474 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021474 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harumiya Satoru Department of Biochemistry, The Cancer Institute, Tokyo, Japanese Foundation for Cancer Research 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170 aut NATIONALLICENCE 700 1- Takeda Kohsuke Department of Biochemistry, The Cancer Institute, Tokyo, Japanese Foundation for Cancer Research 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170 aut NATIONALLICENCE 700 1- Sugiura Tsukasa Laboratory of Biological Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo 183 aut NATIONALLICENCE 700 1- Fukumoto Yuki Laboratory of Biological Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo 183 aut NATIONALLICENCE 700 1- Tachikawa Hiroyuki Laboratory of Biological Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo 183 aut NATIONALLICENCE 700 1- Miyazono Kohei Department of Biochemistry, The Cancer Institute, Tokyo, Japanese Foundation for Cancer Research 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170 aut NATIONALLICENCE 700 1- Fujimoto Daisaburo Laboratory of Biological Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo 183 aut NATIONALLICENCE 700 1- Ichijo Hidenori Department of Biochemistry, The Cancer Institute, Tokyo, Japanese Foundation for Cancer Research 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 745-751 0021-924X 120:4<745 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford