caa a22 4500 397558139 CHVBK 20180308164809.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021475 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021475 The rpoD1 Gene Product Is a Principal Sigma Factor of RNA Polymerase in Microcystis aeruginosa K-81 [Elektronische Daten] [Munehiko Asayama, Hidechika Suzuki, Akio Sato, Tokujiro Aida, Kan Tanaka, Hideo Takahashi, Makoto Shirai] We performed molecular characterization of the RpoD1 protein encoded by the rpoDl gene isolated from a cyanobacterium, Microcystis aeruginosa K-81. The deduced amino acid sequence (416 aa, 48,871 Da) of RpoD1 exhibited extensive similarity to those of proteins of the eubacterial RpoD family (Escherichia coli σ70 homologs). We overproduced and purified RpoDl (54 kDa) from E. coli. Biological and biochemical analyses suggested that RpoDl has a function homologous to that of E. coli σ70 as follows: (i) the RpoD1 protein complemented an rpoD mutant of E. coli strain YN543 (rpoD285) and (ii) the heterologous RNA polymerase holoenzyme reconstituted from the E. coli core enzyme and recombinant RpoDl was specifically transcribed from E. coli promoters. Furthermore, Western blot analysis with antiserum against Synechococcus sp. strain PCC 7942 RpoDl (a principal sigma factor of the σ70 type) indicated that M. aeruginosa K-81 RpoDl (σA1 is the principal σ factor, which is a major component of the σ1 subunit on exponential cell growth. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence cyanobacteria nationallicence Rna polymerase nationallicence sigma factor nationallicence trascription nationallicence Western blot nationallicence Asayama Munehiko Division of Biotechnology, School of Agriculture, Ibaraki University Ami, Inashiki, Ibaraki 300-03 aut Suzuki Hidechika Division of Biotechnology, School of Agriculture, Ibaraki University Ami, Inashiki, Ibaraki 300-03 aut Sato Akio Division of Biotechnology, School of Agriculture, Ibaraki University Ami, Inashiki, Ibaraki 300-03 aut Aida Tokujiro Division of Biotechnology, School of Agriculture, Ibaraki University Ami, Inashiki, Ibaraki 300-03 aut Tanaka Kan Institute of Molecular and Cellular Biosciences, The University of Tokyo Yayoi, Bunkyo-ku, Tokyo 113 aut Takahashi Hideo Institute of Molecular and Cellular Biosciences, The University of Tokyo Yayoi, Bunkyo-ku, Tokyo 113 aut Shirai Makoto Division of Biotechnology, School of Agriculture, Ibaraki University Ami, Inashiki, Ibaraki 300-03 aut The Journal of Biochemistry Oxford University Press 120/4(1996-10), 752-758 0021-924X 120:4<752 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021475 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021475 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Asayama Munehiko Division of Biotechnology, School of Agriculture, Ibaraki University Ami, Inashiki, Ibaraki 300-03 aut NATIONALLICENCE 700 1- Suzuki Hidechika Division of Biotechnology, School of Agriculture, Ibaraki University Ami, Inashiki, Ibaraki 300-03 aut NATIONALLICENCE 700 1- Sato Akio Division of Biotechnology, School of Agriculture, Ibaraki University Ami, Inashiki, Ibaraki 300-03 aut NATIONALLICENCE 700 1- Aida Tokujiro Division of Biotechnology, School of Agriculture, Ibaraki University Ami, Inashiki, Ibaraki 300-03 aut NATIONALLICENCE 700 1- Tanaka Kan Institute of Molecular and Cellular Biosciences, The University of Tokyo Yayoi, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Takahashi Hideo Institute of Molecular and Cellular Biosciences, The University of Tokyo Yayoi, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Shirai Makoto Division of Biotechnology, School of Agriculture, Ibaraki University Ami, Inashiki, Ibaraki 300-03 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/4(1996-10), 752-758 0021-924X 120:4<752 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford