caa a22 4500 397558147 CHVBK 20180308164809.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021263 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021263 The CDw65 Monoclonal Antibodies VIM-8 and VIM-11 Bind to the Neutral Glycolipid V3FucnLc8Cer [Elektronische Daten] [Bernhard Kniep, Jasna Peter-Katalinic, Johannes Miithing, Yasuhiko Komatsu, Otto Majdic, Winfried F. Pickl, Walter Knapp] At the IVth and Vth Workshop on Human Leukocyte Differentiation Antigens a group of monoclonal antibodies recognizing myeloid cells was found to bind to the ganglioside X3- NeuAcVII3FucnLe10Cer (VIM-2 dodecasaccharide). These antibodies were given the provisional cluster of differentiation designation CDw65. Three antibodies of this cluster (VIM-2, VIM-8, and VIM-11) have now been studied In detail at the molecular and the cellular level. Binding of VIM-2 is abolished after treatment of cells with Vibrio cholerae neuramlnidase, whereas VTM-8 and VIM-11 show enhanced binding to neuraminidase-treated cells. We investigated binding of the three mAbs to glycolipid antigens with shorter carbohydrate chains. Distinct differences were observed in the binding of CDw65 antibodies to VIII3- NeuAcVII3FucnLe10Cer (VIM-2 decasaccharide). VIM-2 strongly bound to this antigen, whereas no binding was observed with the other two mAbs. Conversely, the asialoganglioside of the VIM-2 decasaceharide, V3FucnLe8Cer, was not recognized by VIM-2, but this antigen bound strongly VIM-8 and VIM-11. Thus, VIM-2 and the other CDw65 antibodies represented two different antigen specificities. © by the Journal of Biochemistry Regular Papers nationallicence CDw65 nationallicence CDw65s nationallicence VIM-2 nationallicence VIM-8 nationallicence VIM-11 nationallicence Kniep Bernhard Abteilung Zeflbiologie und Immwzokgie, GBF-Gesell8chaft fur Biotechaologische Forschuog mbH Mascheroder W 1, D-38124 Braunschweig, FRG aut Peter-Katalinic Jasna Institut fur Physiologische Chemie der Universitdt Bonn NuBa1Lee 11, D-53115 Bonn, FRG aut Miithing Johannes Institut fur Zellkulturtechnik der Universität Bielefeld Postfach 100131, D-33501 Bielefeld, FRG aut Komatsu Yasuhiko Institut für Immunologie der Universitfit Wien Borschkegasse 8a, A-1090 Wien, Austria aut Majdic Otto Abteilung Zeflbiologie und Immwzokgie, GBF-Gesell8chaft fur Biotechaologische Forschuog mbH Mascheroder W 1, D-38124 Braunschweig, FRG aut Pickl Winfried F. Institut für Immunologie der Universitfit Wien Borschkegasse 8a, A-1090 Wien, Austria aut Knapp Walter Institut für Immunologie der Universitfit Wien Borschkegasse 8a, A-1090 Wien, Austria aut The Journal of Biochemistry Oxford University Press 119/3(1996-03), 456-462 0021-924X 119:3<456 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021263 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021263 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kniep Bernhard Abteilung Zeflbiologie und Immwzokgie, GBF-Gesell8chaft fur Biotechaologische Forschuog mbH Mascheroder W 1, D-38124 Braunschweig, FRG aut NATIONALLICENCE 700 1- Peter-Katalinic Jasna Institut fur Physiologische Chemie der Universitdt Bonn NuBa1Lee 11, D-53115 Bonn, FRG aut NATIONALLICENCE 700 1- Miithing Johannes Institut fur Zellkulturtechnik der Universität Bielefeld Postfach 100131, D-33501 Bielefeld, FRG aut NATIONALLICENCE 700 1- Komatsu Yasuhiko Institut für Immunologie der Universitfit Wien Borschkegasse 8a, A-1090 Wien, Austria aut NATIONALLICENCE 700 1- Majdic Otto Abteilung Zeflbiologie und Immwzokgie, GBF-Gesell8chaft fur Biotechaologische Forschuog mbH Mascheroder W 1, D-38124 Braunschweig, FRG aut NATIONALLICENCE 700 1- Pickl Winfried F. Institut für Immunologie der Universitfit Wien Borschkegasse 8a, A-1090 Wien, Austria aut NATIONALLICENCE 700 1- Knapp Walter Institut für Immunologie der Universitfit Wien Borschkegasse 8a, A-1090 Wien, Austria aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/3(1996-03), 456-462 0021-924X 119:3<456 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford