caa a22 4500 397558163 CHVBK 20180308164809.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021272 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021272 Activation of Detergent-Solubilized Diacylglycerol Acyltransferase by Anionic Phospholipids [Elektronische Daten] [Yasushi Kamisaka, Toro Nakahara] Diacylglycerol acyltransferase (DGAT), which catalyzes the final step in triacylglycerol (TG) biosynthesis, is crucial for lipid accumulation and formation of lipid bodies in an oleaginous fungus, Mortierella ramanniana var. angulispora. Since solubilization of DGAT in the lipid body fraction from this fungus with 0.5% Triton X-100 gave very low recovery of the activity, some activation factors for solubilized DGAT activity were investigated. Addition of phospholipids, especially anionic phospholipids such as phos-phatidic acid and phosphatidylserine, to the assay mixture greatly increased DGAT activity. The activation by these phospholipids was most prominent when 0.2% Triton X-100 was added to the assay mixture. The effect of phosphatidic acid was reproduced using DGAT fraction obtained by 0.5M KC1 elution on Mono S column chromatography. The results provide new insight on activation of DGAT during TG accumulation as well as optimal DGAT assay conditions for solubilized fractions. © by the Journal of Biochemistry Regular Papers nationallicence diacylglycerol nationallicence diacylglycerol acyltransferase nationallicence lipid bodies nationallicence phosphatidic acid nationallicence phosphatidylserine nationallicence Kamisaka Yasushi Applied Microbiology Department, National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki 305 aut Nakahara Toro Applied Microbiology Department, National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki 305 aut The Journal of Biochemistry Oxford University Press 119/3(1996-03), 520-523 0021-924X 119:3<520 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021272 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021272 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kamisaka Yasushi Applied Microbiology Department, National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Nakahara Toro Applied Microbiology Department, National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki 305 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/3(1996-03), 520-523 0021-924X 119:3<520 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford