caa a22 4500 397558171 CHVBK 20180308164809.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021282 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021282 Isolation and Characterization of an Enzyme with β-Glucosidase and β-Fucosidase Activities from Dalbergia cochinchinensis Pierre [Elektronische Daten] [Chantragan Srisomsap, Jisnuson Svasti, Rudee Surarit, Voraratt Champattanachai, Phannee Sawangareetrakul, Kanokporn Boonpuan, Pantipa Subhasitanont, Daranee Chokchaichamnankit] A glycosidase enzyme with both β-glucosidase and β-fucosidase activities has been purified from the seeds of Dalbergia cochinchinensis Pierre (Thai Rosewood) by ammonium sulfate fractionation, preparative isoelectric focusing, and Sephadex G-150 chro-matography. The enzyme has molecular weights of 330,000 in the native state and 66,000 in the denatured state. Hydrolysis of p-NP-β-D-glucoside and p-NP-β-D-fucoside showed pH optimum at pH 5.0 and was inhibited by Rcat-gluconolactone, HgCl2, and p-chloromercuri-benzoate. The Km and fecal values of the purified enzyme were 5.4 mM and 307 s1 for p-NP-β-D-glucoside and 0.54 mM and 151 s−1 for p-NP-β-D-fucoside, so that the latter had by far the higher kcat&Km ratio. p-NP-β-D-galactoside, p-NP-β-D-xyloside, and p-NP-α-L-arabinoside were hydrolyzed more slowly. Hydrolysis of sophorose, laminaribiose, and gentiobiose were also rather slow, and hydrolysis of cellobiose was even slower. No hydrolysis of the cyanogenic glucosides linamarin or prunasin, but some hydrolysis of amygdalin and salicin was found. Further studies are required to identify the natural substrates of the enzyme. However, high yields, ease of purification, and storage stability of the enzyme make it a useful candidate for various applications, such as study of oligosaccharide synthesis by reversal of hydrolysis © by the Journal of Biochemistry Regular Papers nationallicence Dalbergia spp nationallicence β-fucosidase nationallicence β-glucosidase nationallicence glycosidase nationallicence rosewood nationallicence Srisomsap Chantragan Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut Svasti Jisnuson Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut Surarit Rudee Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut Champattanachai Voraratt Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut Sawangareetrakul Phannee Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut Boonpuan Kanokporn Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut Subhasitanont Pantipa Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut Chokchaichamnankit Daranee Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut The Journal of Biochemistry Oxford University Press 119/3(1996-03), 585-590 0021-924X 119:3<585 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021282 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021282 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Srisomsap Chantragan Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut NATIONALLICENCE 700 1- Svasti Jisnuson Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut NATIONALLICENCE 700 1- Surarit Rudee Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut NATIONALLICENCE 700 1- Champattanachai Voraratt Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut NATIONALLICENCE 700 1- Sawangareetrakul Phannee Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut NATIONALLICENCE 700 1- Boonpuan Kanokporn Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut NATIONALLICENCE 700 1- Subhasitanont Pantipa Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut NATIONALLICENCE 700 1- Chokchaichamnankit Daranee Laboratory of Biochemistry, Chulabhorn Research Institute Vibhavadee Rangsit Rd., Bangkok 10210, Thailand aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/3(1996-03), 585-590 0021-924X 119:3<585 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford