caa a22 4500 39755821X CHVBK 20180308164809.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021255 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021255 The Induction of Metachromasia and Circular Dichroism of Coomassie Brilliant Blue R-250 with Collagen and Histone H1 Is Due to the Low Content of Hydrophobic Amino Acid Residues in These Proteins [Elektronische Daten] [Shunji Hattori, Keiko Sakai, Kazuo Watanabe, Tadahiko Fujii] Collagen and histone H1 are stained a pink-red color with Coomassie Brilliant Blue R-250 (Coomassie R) instead of the blue color of most proteins after SDS-PAGE. Spectrophotometrically, this metachromasia was characterized by an increase in the absorbance at 535 nm and a decrease in the absorbance at 600 nm. The ratio of the absorbance at 535 nm to that at 600 nm ranged from 1.5 to 2.5 for the pink-red-stained proteins and was about 1 for the blue stained proteins. In their amino acid composition, the pink-red-stained proteins collagen and histone H1 contained less than 11% of hydrophobic amino acid residues, whereas the five blue-stained proteins examined contained more than 25% of such residues. Collagen and histone H1 also induce circular dichroism (CD) of Coomassie R in the visible region with a different CD spectrum. In the case of native collagen, a CD ( + ) band at 530 nm with 105 molar ellipticity was observed, while the denatured collagen showed a CD (−) band at 530 nm. When the amino groups of the amino acid residues in collagen and histone H1 were converted into hydrophobic groups by fluorescamine treatment, these proteins stained bluer than pink-red and the induced CD was a lower intensity. This is the first report of the metachromatic interaction between a protein and Coomassie R that is accompanied by CD induction. This report also suggests that the induction of metachromasia and CD of Coomassie R was due to the low content of hydrophobic amino acid residues in the peptides. © by the Journal of Biochemistry Regular Papers nationallicence circular dichroism nationallicence collagen nationallicence Coomassie Brilliant Blue nationallicence hydrophobic amino acid residues nationallicence metachromasia nationallicence Hattori Shunji Nippi Research Institute of Biomatrix, Adachi-ku, Tokyo 120 aut Sakai Keiko Nippi Research Institute of Biomatrix, Adachi-ku, Tokyo 120 aut Watanabe Kazuo Nippi Research Institute of Biomatrix, Adachi-ku, Tokyo 120 aut Fujii Tadahiko Nippi Research Institute of Biomatrix, Adachi-ku, Tokyo 120 aut The Journal of Biochemistry Oxford University Press 119/3(1996-03), 400-406 0021-924X 119:3<400 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021255 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021255 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hattori Shunji Nippi Research Institute of Biomatrix, Adachi-ku, Tokyo 120 aut NATIONALLICENCE 700 1- Sakai Keiko Nippi Research Institute of Biomatrix, Adachi-ku, Tokyo 120 aut NATIONALLICENCE 700 1- Watanabe Kazuo Nippi Research Institute of Biomatrix, Adachi-ku, Tokyo 120 aut NATIONALLICENCE 700 1- Fujii Tadahiko Nippi Research Institute of Biomatrix, Adachi-ku, Tokyo 120 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/3(1996-03), 400-406 0021-924X 119:3<400 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford