caa a22 4500 397558252 CHVBK 20180308164809.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021270 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021270 Cloning of cDNA Encoding for a Novel Isozyme of Fructose 6-Phosphate,2-Kinase/Fructose 2,6-Bisphosphatase from Human Placenta [Elektronische Daten] [Akiko Sakai, Mie Kato, Masashi Fukasawa, Masatsune Ishiguro, Eisuke Furuya, Ryuzo Sakakibara] Two independent cDNA clones encoding fructose 6-phosphate,2-kinase/fructose 2,6-bis-phosphatase were isolated from a human placental cDNA library.The deduced amino acid sequences showed that one of the clones, 2K-1, was almost identical to the rat testis isozyme and the other, 2K-3, was different from any known isozymes expressed in mammalian tissues.The results of Southern blot analysis suggested that clones 2K-1 and 2K-3 were encoded as single copy genes and located in different parts of the genome.Since open reading frames of the cDNA clones were notcomplete, we obtained the 5′-end of the clone 2K-3 cDNA using the 5′-rapid amplification of cDNA end method.The entire cDNA (HP; 1,756 bp) had a coding capacity of 519 amino acids (Mr=59,410), and putative phosphorylation sites for protein kinases A and C on the C terminus. Northern blot analysis using a fragment of the HP as a probe showed that a major band of 5.4 kb, significantly different in size from known isozyme mRNAs such as liver (2.1 kb), muscle (1.9 kb), heart (4.0 kb), and testis (2.0 kb), was present in poly(A)+RNA preparations of human first trimester and term placentae.These results strongly suggested that this 5.4 kb mRNA codes a novel isozyme of fructose 6-phosphate,2-kinase/fructose 2,6-bisphosphatase. © by the Journal of Biochemistry Regular Papers nationallicence cDNA cloning nationallicence human placental fructose 6-phosphate nationallicence 2-kinase/fructose 2 nationallicence 6-bisphosphatase nationallicence isozyme nationallicence phosphorylation site nationallicence Sakai Akiko Department of Chemistry, Osaka Medical College, Sawaraki-cho, Takatsuki, Osaka 569 aut Kato Mie Department of Biochemistry, School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852 aut Fukasawa Masashi Department of Preventive Medicine, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852 aut Ishiguro Masatsune Department of Biochemistry, School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852 aut Furuya Eisuke Department of Chemistry, Osaka Medical College, Sawaraki-cho, Takatsuki, Osaka 569 aut Sakakibara Ryuzo Department of Biochemistry, School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852 aut The Journal of Biochemistry Oxford University Press 119/3(1996-03), 506-511 0021-924X 119:3<506 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021270 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021270 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sakai Akiko Department of Chemistry, Osaka Medical College, Sawaraki-cho, Takatsuki, Osaka 569 aut NATIONALLICENCE 700 1- Kato Mie Department of Biochemistry, School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852 aut NATIONALLICENCE 700 1- Fukasawa Masashi Department of Preventive Medicine, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852 aut NATIONALLICENCE 700 1- Ishiguro Masatsune Department of Biochemistry, School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852 aut NATIONALLICENCE 700 1- Furuya Eisuke Department of Chemistry, Osaka Medical College, Sawaraki-cho, Takatsuki, Osaka 569 aut NATIONALLICENCE 700 1- Sakakibara Ryuzo Department of Biochemistry, School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/3(1996-03), 506-511 0021-924X 119:3<506 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford