caa a22 4500 397558279 CHVBK 20180308164809.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021274 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021274 Increased Expression of Carnitine Palmitoyltransferase I Gene Is Repressed by Administering L-Carnitine in the Hearts of Carnitine-Deficient Juvenile Visceral Steatosis Mice [Elektronische Daten] [Rikako Uenaka, Masamichi Kuwajima, Akira Ono, Yuji Matsuzawa, Jun-ichiro Hayakawa, Naohiro Inohara, Yasuo Kagawa, Shigeo Ohta] The juvenile visceral steatosis (JVS) mouse is a novel mutant animal for studying systemic carnitine deficiency. The importance of the model has been pointed out in carnitine-deficient cardiac hypertrophy, since cardiomyopathy has been often improved after oral carnitine therapy in human systemic carnitine deficiency. To understand the effects of carnitine deficiency on gene expression in the heart, we tried to find the genes regulated by carnitine by means of a modified differential display procedure. Carnitine palmitoyltrans-ferase I (CPT I) was one of the isolated genes. The level of CPT I gene expression in the ventricles of the JVS mice was at least three- to sixfold that of normal mice as judged by reverse transcription-polymerase chain reaction (RT-PCR). When the JVS mice were treated with carnitine, CPT I gene expression was repressed to the level of normal mice. Therefore, the increased expression of the CPT I gene was associated with carnitine deficiency © by the Journal of Biochemistry Regular Papers nationallicence cardiac hypertrophy nationallicence carnitine palmitoyltransferase I nationallicence differential display nationallicence gene expression nationallicence systemic carnitine deficiency nationallicence Uenaka Rikako Division of Biochemistry, Institute of Gerontology, Nippon Medical School 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211 aut Kuwajima Masamichi Division of Biochemistry, Institute of Gerontology, Nippon Medical School 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211 aut Ono Akira Department of 2nd Internal Medicine, Faculty of Medicine, Osaka University Suita, Osaka 565 aut Matsuzawa Yuji Department of 2nd Internal Medicine, Faculty of Medicine, Osaka University Suita, Osaka 565 aut Hayakawa Jun-ichiro Institute for Experimental Animals, School of Medicine, Kanazawa University Kanazawa, hhikawa 920 aut Inohara Naohiro Division of Biochemistry, Institute of Gerontology, Nippon Medical School 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211 aut Kagawa Yasuo Department of Biochemistry, Jichi Medical School Mimamikawachi, Tochigi 329-04 aut Ohta Shigeo Division of Biochemistry, Institute of Gerontology, Nippon Medical School 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211 aut The Journal of Biochemistry Oxford University Press 119/3(1996-03), 533-540 0021-924X 119:3<533 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021274 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021274 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Uenaka Rikako Division of Biochemistry, Institute of Gerontology, Nippon Medical School 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211 aut NATIONALLICENCE 700 1- Kuwajima Masamichi Division of Biochemistry, Institute of Gerontology, Nippon Medical School 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211 aut NATIONALLICENCE 700 1- Ono Akira Department of 2nd Internal Medicine, Faculty of Medicine, Osaka University Suita, Osaka 565 aut NATIONALLICENCE 700 1- Matsuzawa Yuji Department of 2nd Internal Medicine, Faculty of Medicine, Osaka University Suita, Osaka 565 aut NATIONALLICENCE 700 1- Hayakawa Jun-ichiro Institute for Experimental Animals, School of Medicine, Kanazawa University Kanazawa, hhikawa 920 aut NATIONALLICENCE 700 1- Inohara Naohiro Division of Biochemistry, Institute of Gerontology, Nippon Medical School 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211 aut NATIONALLICENCE 700 1- Kagawa Yasuo Department of Biochemistry, Jichi Medical School Mimamikawachi, Tochigi 329-04 aut NATIONALLICENCE 700 1- Ohta Shigeo Division of Biochemistry, Institute of Gerontology, Nippon Medical School 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/3(1996-03), 533-540 0021-924X 119:3<533 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford