caa a22 4500 397558295 CHVBK 20180308164809.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021267 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021267 Intra- and Inter-Complex Cross-Linking of Subunits in the Quinol Oxidase Super-Complex from Thermophilic Bacillus PS3 [Elektronische Daten] [Takeshi Tanaka, Masatomo Inoue, Junshi Sakamoto, Nobuhito Sone] Gram-positive thermophilic Bacilli contain quinol-cytochrome c reductase and cyto-chrome c oxidase as two major respiratory complexes of the electron transfer chain, and these enzymes can be extracted with mild detergents as an associated quinol oxidase super-complex.The reductase is composed of three subunits; cytochrome b8 cytochrome c1, and FeS protein, whereas cytochrome c oxidase consists of four subunits numbered 1 through 4. In order to clarify the interactions between the subunits, the super-complex isolated from Bacillus PS3 was cross-linked with three bifunctional cross-linkers; disuccinimidyl tartrate, 3,3′-dithiobis(succinimidylpropionate), and ethylene glycolbis(sulfosuccinimi-dylsuccinate). The most prominent cross-linking was observed for the combination of subunit 1 plus 2 in cytochrome c oxidase, and for that of cytochrome b8 plus cytochrome C1 in the reductase. In addition to these intra-complex cross-linkings, inter-complex linking was observed for the combination of cytochrome b8 plus subunit 1 with ethylene glycolbis-(sulfosuccinimidylsuccinate), and for the combinations of cytochrome b8 plus subunit 1 and cytochrome fit, plus subunit 2 with 3,3 -dithiobis(succinimidylpropionate). Incubation in the presence of Triton X-100, which was confirmed to cleave the two enzyme complexes, selectively reduced the inter-complex cross-linking, suggesting that the chemical cross-linking reflect the spatial arrangement of subunits in the super-complex. © by the Journal of Biochemistry Regular Papers nationallicence chemical cross-linker nationallicence intrinsic membrane protein nationallicence quinol oxidase super-complex nationallicence respiratory chain nationallicence thermophilic bacterium nationallicence Tanaka Takeshi Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Kawazu 680-4, Iizuka, Fukuoka 820 aut Inoue Masatomo Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Kawazu 680-4, Iizuka, Fukuoka 820 aut Sakamoto Junshi Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Kawazu 680-4, Iizuka, Fukuoka 820 aut Sone Nobuhito Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Kawazu 680-4, Iizuka, Fukuoka 820 aut The Journal of Biochemistry Oxford University Press 119/3(1996-03), 482-486 0021-924X 119:3<482 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021267 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021267 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanaka Takeshi Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Kawazu 680-4, Iizuka, Fukuoka 820 aut NATIONALLICENCE 700 1- Inoue Masatomo Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Kawazu 680-4, Iizuka, Fukuoka 820 aut NATIONALLICENCE 700 1- Sakamoto Junshi Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Kawazu 680-4, Iizuka, Fukuoka 820 aut NATIONALLICENCE 700 1- Sone Nobuhito Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Kawazu 680-4, Iizuka, Fukuoka 820 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/3(1996-03), 482-486 0021-924X 119:3<482 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford