caa a22 4500 397558309 CHVBK 20180308164809.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021266 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021266 Time-Dependent Expression of Bone Sialoprotein Fragments in Osteogenesis Induced by Bone Morphogenetic Protein [Elektronische Daten] [Daiji Kobayashi, Hiroko Takita, Morimichi Mizuno, Yasunori Toteuka, Yoshlnori Kuboki] Bone sialoprotein is unique to bone and dentin, but its precise role in these tissues is still unknown, although several hypotheses have been presented. We chose ectopic chondro- and osteogenesis induced by bone morphogenetic protein (BMP) as a model system to examine the role of this protein.Partially purified bovine BMP obtained by a three-step chromato-graphic procedure contained all the active BMPs (natural BMP cocktail). It was combined with insoluble bone matrix and subcutaneously implanted into rats. Expression of bone sialoprotein (BSP) in the implants was followed by using a monoclonal antibody as previously reported. Immunostaining studies showed BSP in the osteoblasts lining the new bone surface at 5 weeks. Western blotting showed 53 and 30 kDa bands, instead of the 57 kDa band normally found in rat femur. These two fragments were metabolically labeled with [3H]proline. The total amount of the fragments rapidly increased after 3 weeks, and at 5 weeks was 3 times as high as that at 2 weeks and still increasing. This time-dependent change was almost parallel to that of osteocalcin. The amount of bone estimated in terms of calcium content increased until 3 weeks and was remained at a plateau thereafter. Alkaline phosphatase activity was prominent only in the first 3 weeks. It was concluded that the 53 and 30 kDa BSP fragments might contribute to maintenance or remodeling in BMP-induced ectopic bone formation. © by the Journal of Biochemistry Regular Papers nationallicence bone morphogenetic protein nationallicence bone remodeling nationallicence bone sialoprotein nationallicence fragmentation nationallicence immunolocalization nationallicence Kobayashi Daiji Department of Oral Surgery II, School of Dentistry, Hokkaido University Kita 13, Nishi 7, Kita-ku, Sapporo 060 aut Takita Hiroko Department of Oral Biochemistry, School of Dentistry, Hokkaido University Kita 13, Nishi 7, Kita-ku, Sapporo 060 aut Mizuno Morimichi Department of Oral Biochemistry, School of Dentistry, Hokkaido University Kita 13, Nishi 7, Kita-ku, Sapporo 060 aut Toteuka Yasunori Department of Oral Surgery II, School of Dentistry, Hokkaido University Kita 13, Nishi 7, Kita-ku, Sapporo 060 aut Kuboki Yoshlnori Department of Oral Biochemistry, School of Dentistry, Hokkaido University Kita 13, Nishi 7, Kita-ku, Sapporo 060 aut The Journal of Biochemistry Oxford University Press 119/3(1996-03), 475-481 0021-924X 119:3<475 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021266 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021266 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kobayashi Daiji Department of Oral Surgery II, School of Dentistry, Hokkaido University Kita 13, Nishi 7, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Takita Hiroko Department of Oral Biochemistry, School of Dentistry, Hokkaido University Kita 13, Nishi 7, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Mizuno Morimichi Department of Oral Biochemistry, School of Dentistry, Hokkaido University Kita 13, Nishi 7, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Toteuka Yasunori Department of Oral Surgery II, School of Dentistry, Hokkaido University Kita 13, Nishi 7, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Kuboki Yoshlnori Department of Oral Biochemistry, School of Dentistry, Hokkaido University Kita 13, Nishi 7, Kita-ku, Sapporo 060 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/3(1996-03), 475-481 0021-924X 119:3<475 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford