caa a22 4500 397558449 CHVBK 20180308164810.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021273 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021273 Induction of the C/EBPβ Gene by Dexamethasone and Glucagon in Primary-Cultured Rat Hepatocytes [Elektronische Daten] [Fumihiko Matsuno, Shoaib Chowdhury, Tomomi Gotoh, Katsuro Iwase, Hiromitsu Matsuzaki, Kiyoshi Takatsuki, Masataka Mori, Masaki Takiguchi] The synthetic glucocorticoid, dexamethasone, and glucagon cooperatively elevated the level of mRNA for the transcription factor CCAAT/enhancer binding protein β(c/EBPβ) in primary-cultured rat hepatocytes. In response to dexamethasone and/or glucagon, c/ EBPβ mRNA started to increase as early as at 30 min, reached a maximum within 2 h, and then gradually decreased. The administration of cycloheximide, a protein synthesis inhibitor, led rather to an increase in c/EBPβ mRNA, which suggested that a labile negative protein factor(s) is involved in regulation of the c/EBPβ mRNA level. Cycloheximide further augmented the increases in c/EBPβ mRNA by dexamethasone and/or glucagon. Therefore, c/EBPβ mRNA accumulation in response to these hormones is apparently independent of ongoing protein synthesis. The elevation of the c/EBPβ mRNA level by these hormones was accounted for by increases in the rate of transcription of the c/EBPβ gene, as deduced on nuclear run-on analysis. Gel mobility shift analysis revealed that the DNA-binding activity of C/EBPβ was increased cooperatively by dexamethasone and glucagon. These results suggest that the c/EBPβ gene is primarily induced by glucocorticoids and/or glucagon and that the accumulated c/EBPβ protein is then involved in secondary activation of target genes in response to these hormones in the liver © by the Journal of Biochemistry Regular Papers nationallicence C/EBPβ nationallicence dexamethasone nationallicence glucagon nationallicence liver nationallicence primary hepatocytes nationallicence Matsuno Fumihiko Departments of Molecular Genetics, Kvmamoto University School of Medicine Kumamoto 862 aut Chowdhury Shoaib Departments of Molecular Genetics, Kvmamoto University School of Medicine Kumamoto 862 aut Gotoh Tomomi Departments of Molecular Genetics, Kvmamoto University School of Medicine Kumamoto 862 aut Iwase Katsuro Departments of Molecular Genetics, Kvmamoto University School of Medicine Kumamoto 862 aut Matsuzaki Hiromitsu Internal Medicine, Kvmamoto University School of Medicine Kumamoto 862 aut Takatsuki Kiyoshi Internal Medicine, Kvmamoto University School of Medicine Kumamoto 862 aut Mori Masataka Departments of Molecular Genetics, Kvmamoto University School of Medicine Kumamoto 862 aut Takiguchi Masaki Departments of Molecular Genetics, Kvmamoto University School of Medicine Kumamoto 862 aut The Journal of Biochemistry Oxford University Press 119/3(1996-03), 524-532 0021-924X 119:3<524 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021273 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021273 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsuno Fumihiko Departments of Molecular Genetics, Kvmamoto University School of Medicine Kumamoto 862 aut NATIONALLICENCE 700 1- Chowdhury Shoaib Departments of Molecular Genetics, Kvmamoto University School of Medicine Kumamoto 862 aut NATIONALLICENCE 700 1- Gotoh Tomomi Departments of Molecular Genetics, Kvmamoto University School of Medicine Kumamoto 862 aut NATIONALLICENCE 700 1- Iwase Katsuro Departments of Molecular Genetics, Kvmamoto University School of Medicine Kumamoto 862 aut NATIONALLICENCE 700 1- Matsuzaki Hiromitsu Internal Medicine, Kvmamoto University School of Medicine Kumamoto 862 aut NATIONALLICENCE 700 1- Takatsuki Kiyoshi Internal Medicine, Kvmamoto University School of Medicine Kumamoto 862 aut NATIONALLICENCE 700 1- Mori Masataka Departments of Molecular Genetics, Kvmamoto University School of Medicine Kumamoto 862 aut NATIONALLICENCE 700 1- Takiguchi Masaki Departments of Molecular Genetics, Kvmamoto University School of Medicine Kumamoto 862 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/3(1996-03), 524-532 0021-924X 119:3<524 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford