caa a22 4500 397558457 CHVBK 20180308164810.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021496 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021496 Expression of Cadherin-Catenin Complexes in Human Leukemia Cell Lines [Elektronische Daten] [Jun-ichiro Tsutsui, Maiko Moriyama, Naomichi Arima, Hindeo Ohtsubo, Hiromitsu Tanaka, Massyuki Ozawa] Cadherins are Ca2+-dependent cell-cell adhesion molecules, and are involved in the formation and maintenance of the histo-architecture. Using cultured human leukemia cell lines (adult T cell leukemia and thymus-derived lymphoma cell lines), we obtained evidence that cadherins and catenins are expressed in these cell lines but not in normal leukocytes. Immunoblot analysis of cells using a pan-cadherin serum, directed against the conserved carboxyl-terminus of cadherins, revealed a major band of 130 kDa and a minor one of 135 kDa. The 130 kDa cadherin was also recognized by anti-N-cadherin antibodies. A human N-cadherin cDNA probe hybridized to a 4.3 kb mRNA isolated from cells immunologically positive for N-cadherin. Sequencing of the cDNA fragments isolated from the cells revealed a N-cadherin. sequence. Cell surface expression of N-cadherin was confirmed by indirect immunofluorescence staining of the cells. Immunoblot and Northern blot analyses also revealed the presence of α-catenin, β-catenin, and γ-catenin (plakoglobin) in these cell lines. Immunoprecipitation with anti-N-cadherin antibodies and subsequent immunoblot analysis with anti-catenin antibodies revealed that N-cadherin is associated with α- and β-catenins, a prerequisite for cadherins to be functional. These results suggest an important role of the cadherin-catenin complexes in the behavior of the leukemia cells. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence ATL nationallicence N-cadherin nationallicence α-catenin nationallicence β-catenin nationallicence leukemia nationallicence Tsutsui Jun-ichiro Department of Biochemistry Facult of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890 aut Moriyama Maiko Department of Biochemistry Facult of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890 aut Arima Naomichi lst Dapartnment of Medicine Faculty of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890 aut Ohtsubo Hindeo lst Dapartnment of Medicine Faculty of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890 aut Tanaka Hiromitsu lst Dapartnment of Medicine Faculty of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890 aut Ozawa Massyuki Department of Biochemistry Facult of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890 aut The Journal of Biochemistry Oxford University Press 120/5(1996-11), 1034-1039 0021-924X 120:5<1034 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021496 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021496 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsutsui Jun-ichiro Department of Biochemistry Facult of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890 aut NATIONALLICENCE 700 1- Moriyama Maiko Department of Biochemistry Facult of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890 aut NATIONALLICENCE 700 1- Arima Naomichi lst Dapartnment of Medicine Faculty of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890 aut NATIONALLICENCE 700 1- Ohtsubo Hindeo lst Dapartnment of Medicine Faculty of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890 aut NATIONALLICENCE 700 1- Tanaka Hiromitsu lst Dapartnment of Medicine Faculty of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890 aut NATIONALLICENCE 700 1- Ozawa Massyuki Department of Biochemistry Facult of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/5(1996-11), 1034-1039 0021-924X 120:5<1034 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford