caa a22 4500 397558473 CHVBK 20180308164810.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021503 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021503 Effect of Aluminum Ion on Fe2+-Induced Lipid Peroxidation in Phospholipid Liposomes under Acidic Conditions [Elektronische Daten] [Takao Ohyashiki, Tomokazu Karino, Satomi Suzuki, Katsuhiko Matsui] The effects of Al3+ on Fe2+-induced lipid peroxidation in phospholipid liposomes consisting of phosphatidylcholine (PC) and phosphatidylserine (PS) were examined under acidic conditions. The stimulatory effect of Al3+ on Fe2+-induced lipid peroxidation in the liposomes showed a biphasic response against pH variation, and the maximum stimulation was observed around pH 6.0. In addition, it was found that the stimulatory effect of Al3+ on the lipid peroxidation was dependent on the proportion of PS in the liposomes. On the other hand, the lipid peroxidation in PC liposomes was not stimulated by the addition of Al3+. From these findings, it is suggested that the Al3+ effect on Fe2+-induced lipid peroxidation under acidic conditions is largely dependent on the phospholipid composition. Trivalent cations such as Tb3+ and Ga3+ also stimulated Fe2+-induced lipid peroxidation in PC/PS liposomes under acidic conditions, but divalent cations (Zn2+ and Mn2+) showed no stimulatory effect. The extents of Fe2+ disappearance and Fe3+ formation during the reaction were enhanced by the addition of Al3+ or Ga2+, but Th3 + had no effect on Fe2+ disappearance. The results with 1,6-diphenyl-1,3,5-hexatriene (DPH) showed that the fluorescence anisotropy of DPH-labeled PC/PS liposomes under acidic conditions was increased by the addition of Al3+. Furthermore, there is a relation between the extents of the fluorescence anisotropy of the complex and TBARS production. In contrast, the fluorescence anisotropy of DPH molecules embedded in PC liposomes was not changed by the addition of Al3+. Based on these results, a possible mechanism of the stimulatory effect of Al3+ on Fe2+-induced lipid peroxidation under acidic conditions is discussed. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence acidosis nationallicence Al3+ effect nationallicence lipid peroxidation nationallicence phospholipid liposome nationallicence Ohyashiki Takao Department of Clinical Chemistry aut Karino Tomokazu Department of Clinical Chemistry aut Suzuki Satomi Department of Clinical Chemistry aut Matsui Katsuhiko Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanagawa-Machi Kanazawa, Ishikawa 920-11 aut The Journal of Biochemistry Oxford University Press 120/5(1996-11), 895-900 0021-924X 120:5<895 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021503 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021503 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohyashiki Takao Department of Clinical Chemistry aut NATIONALLICENCE 700 1- Karino Tomokazu Department of Clinical Chemistry aut NATIONALLICENCE 700 1- Suzuki Satomi Department of Clinical Chemistry aut NATIONALLICENCE 700 1- Matsui Katsuhiko Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanagawa-Machi Kanazawa, Ishikawa 920-11 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/5(1996-11), 895-900 0021-924X 120:5<895 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford