caa a22 4500 397558481 CHVBK 20180308164810.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021507 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021507 Muscle-Type Tropomyosin of Sea Urchin Egg Increases the Actin-Binding of Nonmuscie-Type Tropomyosin [Elektronische Daten] [Takayuki Tobita, Fumiko Hiraide, Jun-Ichi Miyazaki, Tadashi Ishimoda-Takagi] Tropomyosin isoforms in eggs of several species of sea urchins are classified into two types, muscle-type and nomnuscle-type, based on their antigenicities. Their actin-binding abilities were investigated using muscle-type isoform (32K) and nonmuscie-type isoform (30K), which were purified by the method previously reported and separated by isoelectric focusing from eggs of sea urchin, Strongylocentrotus intermedius. Co-sedimentation assays revealed that 32K could stoichiometrically bind to actin filaments independently of the 30K, but 30K alone bound very poorly. The actin-binding of 30K was, however, considerably increased in the presence of 32K, and the molar ratio of the bound 30K and 32K was approximately 1:1. The increase in the actin-binding of 30K is probably caused by the interaction of 30K with 32K in a head-to-tail manner, as indicated by the higher specific viscosity of the mixture than that of 32K alone. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence actin-binding ability nationallicence head-to-tail interaction nationallicence muscle-type isoform nationallicence non-muscle-type isoform nationallicence sea urchin egg tropomyosin nationallicence Tobita Takayuki Institute of Biological Sciences, University of Tsukuba Tennoudai 1-1-1, Tsukuba, Ibaraki 305 aut Hiraide Fumiko Department of Biology, Tokyo Gakugei University Koganei, Tokyo 184 aut Miyazaki Jun-Ichi Institute of Biological Sciences, University of Tsukuba Tennoudai 1-1-1, Tsukuba, Ibaraki 305 aut Ishimoda-Takagi Tadashi Department of Biology, Tokyo Gakugei University Koganei, Tokyo 184 aut The Journal of Biochemistry Oxford University Press 120/5(1996-11), 922-928 0021-924X 120:5<922 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021507 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021507 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tobita Takayuki Institute of Biological Sciences, University of Tsukuba Tennoudai 1-1-1, Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Hiraide Fumiko Department of Biology, Tokyo Gakugei University Koganei, Tokyo 184 aut NATIONALLICENCE 700 1- Miyazaki Jun-Ichi Institute of Biological Sciences, University of Tsukuba Tennoudai 1-1-1, Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Ishimoda-Takagi Tadashi Department of Biology, Tokyo Gakugei University Koganei, Tokyo 184 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/5(1996-11), 922-928 0021-924X 120:5<922 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford