caa a22 4500 39755849X CHVBK 20180308164810.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021517 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021517 Genomic Structures and Characterization of Rael Family Members Encoding GPI-Anchored Cell Surface Proteins and Expressed Predominantly in Embryonic Mouse Brain [Elektronische Daten] [Midori Nomura, Zhihua Zou, Tadashi Joh, Yoshihiro Takihara, Yoichi Matsuda, Kazunori Shimada] Rae1 α, Rae1β, and Rae1γ cDNAs isolated from retinoic acid-treated mouse embryonal carcinoma F9 cells encode cell surface proteins sharing partial homology with MHC class I molecules, and mRNAs corresponding to these cDNAs were detected exclusively in early mouse embryos, especially in the head region. To initiate studies on their roles, the rae1α gene and the genomic DNAs covering the complete coding regions of the rae1β and rae1γ genes were isolated and their structures were analyzed. Although the coding regions of the three rae1 genes were highly homologous, the restriction map of the 5′-end region of the rae1α gene differed from that of the rae1β and rae1γ genes. The rae1 family members were mapped by FISH on mouse chromosome 10A4 region. Genomic DNAs hybridizable with a Rae1 cDNA were not detected in rat and human. Rae1 genes were preferentially expressed in early mouse embryos, preferentially in the brain, and RAE1 proteins were anchored on the cell surface by a glycosyl phosphatidylinositol (GPI)-tail, a feature shared by important cell surface ligands. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence cell surface protein nationallicence early mouse embryo nationallicence P450 nationallicence GPI-anchor nationallicence Rae1 nationallicence Nomura Midori Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut Zou Zhihua Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut Joh Tadashi Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut Takihara Yoshihiro Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut Matsuda Yoichi Laboratory of Animal Genetics, School of Agricultural Sciences, Nagoya University Chikusa-ku, Nagoya 464-01 aut Shimada Kazunori Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut The Journal of Biochemistry Oxford University Press 120/5(1996-11), 987-995 0021-924X 120:5<987 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021517 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021517 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nomura Midori Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Zou Zhihua Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Joh Tadashi Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Takihara Yoshihiro Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Matsuda Yoichi Laboratory of Animal Genetics, School of Agricultural Sciences, Nagoya University Chikusa-ku, Nagoya 464-01 aut NATIONALLICENCE 700 1- Shimada Kazunori Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/5(1996-11), 987-995 0021-924X 120:5<987 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford