caa a22 4500 397558511 CHVBK 20180308164810.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021508 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021508 Isolation and Characterization of the Human Chromosomal Gene for Prostacyclin-Stimulating Factor [Elektronische Daten] [Seiichi Mizushima, Hironori Sato, Takaaki Negishi, Hiroko Koushima, Atsushi Okamoto, Atsushi Nii, Toshihiko Hashimoto, Fumio Umeda, Hajime Nawata, Toshinori Kanamori] Prostacyclin-stimulating factor (PSF) is a protein which acts on vascular endothelial cells and stimulates the production of prostacyclin. Recently, we were able to purify PSF from the conditioned medium of cultured human diploid fibroblasts and clone PSF cDNA. In this study, we screened a human genomic library and isolated genomic clones to determine the structure of the human chromosomal PSF gene. By determining the nucleotide sequence and transcription initiation site of this gene, we found that it comprises 5 exons and 4 introns. Southern hybridization analysis indicated the presence of a single copy of the PSF gene per haploid set of chromosomes. The 300 bp upstream of the transcription initiation site had a very high GC content, and 7 binding sites for the transcription regulating factor Sp1 were present. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence exon-intron structure nationallicence genomic DNA nationallicence promoter structure nationallicence prostacyclin (PGI2)-stimulating factor nationallicence transcription initiation site nationallicence Mizushima Seiichi Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut Sato Hironori Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut Negishi Takaaki Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut Koushima Hiroko Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut Okamoto Atsushi Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut Nii Atsushi Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut Hashimoto Toshihiko Third Department of Internal Medicine, Faculty of Medicine, Kyushu University Maidashi 3-1-1, Higashi-ku, Fukuoka 812 aut Umeda Fumio Third Department of Internal Medicine, Faculty of Medicine, Kyushu University Maidashi 3-1-1, Higashi-ku, Fukuoka 812 aut Nawata Hajime Third Department of Internal Medicine, Faculty of Medicine, Kyushu University Maidashi 3-1-1, Higashi-ku, Fukuoka 812 aut Kanamori Toshinori Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut The Journal of Biochemistry Oxford University Press 120/5(1996-11), 929-933 0021-924X 120:5<929 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021508 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021508 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mizushima Seiichi Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut NATIONALLICENCE 700 1- Sato Hironori Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut NATIONALLICENCE 700 1- Negishi Takaaki Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut NATIONALLICENCE 700 1- Koushima Hiroko Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut NATIONALLICENCE 700 1- Okamoto Atsushi Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut NATIONALLICENCE 700 1- Nii Atsushi Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut NATIONALLICENCE 700 1- Hashimoto Toshihiko Third Department of Internal Medicine, Faculty of Medicine, Kyushu University Maidashi 3-1-1, Higashi-ku, Fukuoka 812 aut NATIONALLICENCE 700 1- Umeda Fumio Third Department of Internal Medicine, Faculty of Medicine, Kyushu University Maidashi 3-1-1, Higashi-ku, Fukuoka 812 aut NATIONALLICENCE 700 1- Nawata Hajime Third Department of Internal Medicine, Faculty of Medicine, Kyushu University Maidashi 3-1-1, Higashi-ku, Fukuoka 812 aut NATIONALLICENCE 700 1- Kanamori Toshinori Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd. 1-1 Kamiya 1-chome, Kita-ku, Tokyo 115 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/5(1996-11), 929-933 0021-924X 120:5<929 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford