caa a22 4500 397558570 CHVBK 20180308164810.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021501 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021501 Unfolding and Refolding of the N-Terminal Fragments of Staphylococcal Nuclease R in Guanidine Hydrochloride [Elektronische Daten] [Bo Zhou, Guo-Zhong Jing] The conformational and activity changes of a family of peptide fragments of staphylococcal nuclease R, which extend from residues −6 to 102, −6 to 110, −6 to 121, −6 to 135, and −6 to 141, during unfolding and refolding in different concentrations of guanidine hydrochloride have been studied. The studies indicate that the conformational stability in guanidine hydrochloride solution of the N-terminal fragment increases with increasing chain length, and that interaction and recognition between amino acid residues which are related to formation of the native conformation also increase with growth of the peptide chain, but such interaction becomes effective only when the polypeptide chain reaches a certain length. The changes in conformation and catalytic activity of the N-terminal fragments during unfolding and refolding demonstrate that conformational adjustments are necessary during chain elongation to generate the native conformation of a biologically active protein. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence N-terminal fragment nationallicence nuclease nationallicence peptide folding nationallicence unfolding and refolding nationallicence staphhylococcus nationallicence Zhou Bo National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing 100101, China aut Jing Guo-Zhong National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing 100101, China aut The Journal of Biochemistry Oxford University Press 120/5(1996-11), 881-888 0021-924X 120:5<881 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021501 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021501 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Bo National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing 100101, China aut NATIONALLICENCE 700 1- Jing Guo-Zhong National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing 100101, China aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/5(1996-11), 881-888 0021-924X 120:5<881 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford