caa a22 4500 397558600 CHVBK 20180308164810.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021495 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021495 Protein-RNA and Protein-Protein Interactions of the Drosophila Sex-Lethal Mediated by Its RNA-Binding Domains [Elektronische Daten] [Eiji Sakashita, Hiroshi Sakamoto] The Drosophila Sex-lethal (Sxl) contains two RNA-binding domains (RBDs) which belong to the RNA recognition motif (RRM) group. Sxl binds to a specific uridine-rich sequence which is believed to be the major cis-acting element for the splicing regulation of the transformer (tra) mRNA precursor. Here we show evidence supporting the previous suggestion that Sxl recognizes the sequence context downstream of the uridine-rich sequence. In addition, by means of UV-crosslinking assays with Sxl deletion constructs, we have demonstrated that Sxl RNA binding requires both of its RBDs for specificity and strength. Moreover, by the yeast two-hybrid analysis, we found that homodimeric interaction occurs between two Sxl molecules. Interestingly, the amino- and carboxy-terminal regions outside of the Sxl RBDs are dispensable for such dimerization, indicating that the protein-protein interaction is also mediated by RBDs. Coprecipitation experiments in vitro showed that the protein-protein interaction seems to be RNA-dependent but greatly enhanced by addition of the specific RNA containing the Sxl binding site, suggesting that the conformational change which is induced on binding to RNA may facilitate the interaction between Sxl molecules. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence protein-protein interaction nationallicence RNA-binding domain (RBD) nationallicence RNA recognition motif (RRM) nationallicence Sex-lethal (Sxl) nationallicence two-hybrid analysis nationallicence Sakashita Eiji Department of Biology, Faculty of Science, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657 aut Sakamoto Hiroshi Department of Biology, Faculty of Science, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657 aut The Journal of Biochemistry Oxford University Press 120/5(1996-11), 1028-1033 0021-924X 120:5<1028 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021495 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021495 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sakashita Eiji Department of Biology, Faculty of Science, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657 aut NATIONALLICENCE 700 1- Sakamoto Hiroshi Department of Biology, Faculty of Science, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/5(1996-11), 1028-1033 0021-924X 120:5<1028 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford