caa a22 4500 397558635 CHVBK 20180308164810.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021514 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021514 Cloning, Sequence Analysis, and Expression in Escherichia coli of the Gene Encoding the Candida utilis Urate Oxidase (Uricase) [Elektronische Daten] [Yasuji Koyama, Toshio Ichikawa, Eiichi Nakano] A urate oxidase (uricase) gene was cloned from Candida utilis with an oligonucleotide probe based on the amino acid sequence of cyanogen bromide-cleaved uricase. The uricase gene contains 909 base pairs and encodes a protein with a predicted mass of 34,193 Da. Candida uricase was similar (49% match in amino acid sequence) to the uricase from Aspergillus flavus. The uricase from Candida utilis has four cysteines and one of them, Cysl68, participates in the enzyme activity. This enzyme was expressed to a level of about 20% of total cellular protein in an Escherichia coli cell as a soluble and functional form. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence Candida utilis nationallicence nucleotide sequence analysis nationallicence overexpression inE. coli nationallicence oxidation of cysteine residue nationallicence uricase nationallicence Koyama Yasuji Research and Development Division, Kikkoman Corporation, Noda 399, Noda, Chiba 278 aut Ichikawa Toshio Research and Development Division, Kikkoman Corporation, Noda 399, Noda, Chiba 278 aut Nakano Eiichi Research and Development Division, Kikkoman Corporation, Noda 399, Noda, Chiba 278 aut The Journal of Biochemistry Oxford University Press 120/5(1996-11), 969-973 0021-924X 120:5<969 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021514 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021514 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koyama Yasuji Research and Development Division, Kikkoman Corporation, Noda 399, Noda, Chiba 278 aut NATIONALLICENCE 700 1- Ichikawa Toshio Research and Development Division, Kikkoman Corporation, Noda 399, Noda, Chiba 278 aut NATIONALLICENCE 700 1- Nakano Eiichi Research and Development Division, Kikkoman Corporation, Noda 399, Noda, Chiba 278 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/5(1996-11), 969-973 0021-924X 120:5<969 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford