caa a22 4500 39755866X CHVBK 20180308164810.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021499 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021499 Identification of Interleukin-6 as a Factor That Induces Neurite Outgrowth by PC12 Cells Primed with NGF [Elektronische Daten] [Sayoka Ihara, Akihiro Iwamatsu, Toshihide Fujiyoshi, Akiko Komi, Takao Yamori, Yasuhisa Fukui] We have screened for a factor that induces neurite outgrowth by PC12 cells only after NGF pretreatment in the supernatants of 38 transformed cell lines, finding three positive clones. The factor showing the strongest activity was purified and identified as IL6. In this NGF-IL6 system, the process of neurite outgrowth by PC12 cells can be separated into NGF-dependent and IL6-dependent steps. The IL6-dependent step requires RNA synthesis, suggesting that IL6 induces new gene expression depending on NGF-priming. These findings suggest that the gene expression during the differentiation process is regulated by at least two signals. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Rapid Communication nationallicence differentiation nationallicence IL6 nationallicence neurite nationallicence NGF nationallicence PC12 cells nationallicence Ihara Sayoka Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Division of Agriculture and Life Science The University of Tokyo 1-1-1 Yayoi-cho, Bunkyo-ku, Tokyo 113 aut Iwamatsu Akihiro Central Laboratories for Key Technology, Kirin Brewery Co., Ltd. 1-13-5 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236 aut Fujiyoshi Toshihide Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Division of Agriculture and Life Science The University of Tokyo 1-1-1 Yayoi-cho, Bunkyo-ku, Tokyo 113 aut Komi Akiko Japanese Foundation for Cancer Research 1-37-1 Kamiikebukuro, Toshima-ku, Tokyo 170 aut Yamori Takao Japanese Foundation for Cancer Research 1-37-1 Kamiikebukuro, Toshima-ku, Tokyo 170 aut Fukui Yasuhisa Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Division of Agriculture and Life Science The University of Tokyo 1-1-1 Yayoi-cho, Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 120/5(1996-11), 865-868 0021-924X 120:5<865 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021499 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021499 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ihara Sayoka Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Division of Agriculture and Life Science The University of Tokyo 1-1-1 Yayoi-cho, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Iwamatsu Akihiro Central Laboratories for Key Technology, Kirin Brewery Co., Ltd. 1-13-5 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236 aut NATIONALLICENCE 700 1- Fujiyoshi Toshihide Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Division of Agriculture and Life Science The University of Tokyo 1-1-1 Yayoi-cho, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Komi Akiko Japanese Foundation for Cancer Research 1-37-1 Kamiikebukuro, Toshima-ku, Tokyo 170 aut NATIONALLICENCE 700 1- Yamori Takao Japanese Foundation for Cancer Research 1-37-1 Kamiikebukuro, Toshima-ku, Tokyo 170 aut NATIONALLICENCE 700 1- Fukui Yasuhisa Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Division of Agriculture and Life Science The University of Tokyo 1-1-1 Yayoi-cho, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/5(1996-11), 865-868 0021-924X 120:5<865 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford