caa a22 4500 39756614X CHVBK 20180308164828.0 cr unu---uuuuu 161202e19960403xx s 000 0 eng 10.1093/jnci/88.7.450 doi (NATIONALLICENCE)oxford-10.1093/jnci/88.7.450 Deletion and Translocation Involving Chromosome 3(p14) in Two Tumorigenic Kaposi's Sarcoma Cell Lines [Elektronische Daten] [Nicholas C. Popescu, Drazen B. Zimonjic, Sophie Leventon-Kriss, Joseph L. Bryant, Yanto Lunardi-Iskandar, Robert C. Gallo] Background Two neoplastic Kaposi's sarcoma (KS) cell lines, KS Y-1 (derived from a patient with KS associated with acquired immunodeficiency syndrome) and KS SLK (derived from an immunosuppressed patient with a renal transplant and KS or iatrogenic KS), have been shown to have abnormal chromosome constitution and to require no exogenous growth factors. They produce malignant tumors in immunodeficient mice. In contrast, all other cell cultures prepared in the past from KS specimens have been shown to have normal diploid characteristics, are hyperplastic, and depend on cytokines for growth, but they do not produce malignant tumors in immunodeficient mice. Purpose We investigated whether the chromosomal changes that occurred in these KS cell lines were random or nonrandom and if such changes contribute to the pathogenesis of KS. Methods We used the conventional G-banding technique and fluorescence in situ hybridization to identify structural and numerical chromosomal changes in the KS cell lines. Results We demonstrated that both cell lines are aneuploid and have some additional features in common, i.e., loss of copies of chromosomes 14 and 21 and nonrandom translocations and deletions in the short arm of chromosome 3 at region 3p14. These KS cell lines also exhibit loss of heterozygosity of loci at region 3p14-ter. Conclusion This is the first time nonrandom chromosomal alterations have been described in KS neoplastic cells. On the basis of information available on other cancers, the chromosome 3 alterations observed here can be expected to contribute to the neoplastic process in KS. Implications Future research should focus on the identification of cytogenetic markers, thus facilitating generation of specific molecular probes for detecting neoplastic cells early in the disease process. [J Natl Cancer Inst 1996;88:450-5] © Oxford University Press REPORTS nationallicence Popescu Nicholas C. Laboratory of Biology, Division of Basic Sciences, National Cancer Institute Bethesda, MD aut Zimonjic Drazen B. Laboratory of Biology, Division of Basic Sciences, National Cancer Institute Bethesda, MD aut Leventon-Kriss Sophie Central Virus Laboratory, Chaim Sheba Medical Center, Tel-Aviv University, School of Medicine, Tel-Hashomer Israel aut Bryant Joseph L. Animal Care Unit, National Institute of Dental Research Bethesda, MD aut Lunardi-Iskandar Yanto Laboratory of Tumor Cell Biology, Division of Basic Sciences, National Cancer Institute Bethesda, MD aut Gallo Robert C. Laboratory of Tumor Cell Biology, Division of Basic Sciences, National Cancer Institute Bethesda, MD aut JNCI: Journal of the National Cancer Institute Oxford University Press 88/7(1996-04-03), 450-455 0027-8874 88:7<450 1996 88 jnci https://doi.org/10.1093/jnci/88.7.450 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jnci/88.7.450 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Popescu Nicholas C. Laboratory of Biology, Division of Basic Sciences, National Cancer Institute Bethesda, MD aut NATIONALLICENCE 700 1- Zimonjic Drazen B. Laboratory of Biology, Division of Basic Sciences, National Cancer Institute Bethesda, MD aut NATIONALLICENCE 700 1- Leventon-Kriss Sophie Central Virus Laboratory, Chaim Sheba Medical Center, Tel-Aviv University, School of Medicine, Tel-Hashomer Israel aut NATIONALLICENCE 700 1- Bryant Joseph L. Animal Care Unit, National Institute of Dental Research Bethesda, MD aut NATIONALLICENCE 700 1- Lunardi-Iskandar Yanto Laboratory of Tumor Cell Biology, Division of Basic Sciences, National Cancer Institute Bethesda, MD aut NATIONALLICENCE 700 1- Gallo Robert C. Laboratory of Tumor Cell Biology, Division of Basic Sciences, National Cancer Institute Bethesda, MD aut NATIONALLICENCE 773 0- JNCI: Journal of the National Cancer Institute Oxford University Press 88/7(1996-04-03), 450-455 0027-8874 88:7<450 1996 88 jnci Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford