caa a22 4500 397566875 CHVBK 20180308164830.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.3382/ps.0751508 doi (NATIONALLICENCE)oxford-10.3382/ps.0751508 Influence of Vitamins E and C on the Toxic Effects of Ochratoxin A and T-2 Toxin in Chicks [Elektronische Daten] [DIRK HOEHLER, RONALD R. MARQUARDT] The objective of this study was to determine whether two antioxidant vitamins, vitamins E and C, were able to counteract the production of lipid peroxides and the corresponding toxic signs of two important but diverse mycotoxins, T-2 toxin and ochratoxin A (OA). Experiment 1 was designed in a 3 × 3 factorial arrangement using three doses of vitamin E (dl-α-tocopheryl acetate) in the diet of Leghorn cockerels (required level according to NRC, l0×, and l00× requirements) and three toxin treatments [no toxin (Diets 1, 2, and 3), 4 mg T-2/kg of diet (Diets 4, 5, and 6), and 2.5 mg OA/kg of diet (Diets 7, 8, and 9)]. The experimental design for Experiment 2 was the same as for Experiment 1 except that Vitamin C (0, 200, and 1,000 mg/kg of diet) was used in place of vitamin E and the concentration of T-2 in Diets 4, 5, and 6 was increased to 5 mg/kg of diet. Six replicates were used per treatment with four birds per replicate. In both experiments, OA and T-2 decreased the performance of the chicks significantly. The concentration of uric acid in the plasma increased (P < 0.001) when OA was added to the diet, whereas the supplementation of the diet with vitamin E (l00× the requirement) partially counteracted this effect (P = 0.07). The presence of T-2, and especially OA, in the diet decreased the concentration of α-tocopherol in the liver (P < 0.001). Consistent with these findings were increased values of malondialdehyde (MDA) in the liver due to OA. In Experiment 1, vitamin E supplementation partially ameliorated the prooxidative effects of OA by decreasing the concentrations of MDA (P < 0.05). These data suggest that lipid peroxides are formed in vivo by T-2 and especially by OA and that these effects can be partially counteracted by an antioxidant such as vitamin E but not by vitamin C. © 1996 Poultry Science Association, Inc. Metabolism and Nutrition nationallicence ochratoxin A nationallicence T-2 toxin nationallicence lipid peroxidation nationallicence antioxidants nationallicence malondialdehyde nationallicence HOEHLER DIRK aut MARQUARDT RONALD R. 3To whom correspondence should be addressed. aut Poultry Science Oxford University Press 75/12(1996-12), 1508-1515 0032-5791 75:12<1508 1996 75 ps https://doi.org/10.3382/ps.0751508 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0751508 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- HOEHLER DIRK aut NATIONALLICENCE 700 1- MARQUARDT RONALD R. 3To whom correspondence should be addressed aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/12(1996-12), 1508-1515 0032-5791 75:12<1508 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford