caa a22 4500 397566964 CHVBK 20180308164830.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.3382/ps.0750321 doi (NATIONALLICENCE)oxford-10.3382/ps.0750321 Modeling Residue Uptake by Eggs [Elektronische Daten] 1. Similar Drug Residue Patterns in Developing Yolks Following Injection with Ampicillin or Oxytetracycline [DAN J. DONOGHUE, HERMAN HAIRSTON, STUART A. GAINES, MARY J. BARTHOLOMEW, ANN M. DONOGHUE] This study was conducted to model the pattern of antibiotic drug uptake within yolks of developing follicles. In two separate experiments, 16 hens were divided into equal groups (n = 8) and injected only once with either 400 mg/kg ampicillin or 200 mg/kg oxytetracycline (OTC: total hens = 32) approximately 1 h after oviposition. Twenty-four hours following injections, hens were euthanatized and the ovaries were collected. Yolks were dissected free from the individual follicles with a blunt probe. Individual large yellow yolks (≥ 0.2 g) and a pool of 5 small yellow yolks (< 0.2 g) were collected for determination of ampicillin or OTC content. Samples were prepared and assayed using an agar diffusion microbiological method. Selected parameters were not different (P > 0.05) between Experiments 1 and 2 and the data were combined. Results indicate that short-term drug exposure in hens produced incorporation of drug residues in developing yolks in a specific pattern that does not appear to be drug dependent (P > 0.05). These incurred residues are contained in developing yolks that are days to weeks from being ovulated. Drug residues were greater (total microgram content) in some of the less mature yolks vs the largest preovulatory yolk. This may lead to a sequential release of eggs with increasing residue content, even after drug withdrawal. These data were used to construct a model to predict the pattern of incurred residues in formed eggs following a hen's exposure to drugs or other contaminants. © 1996 Poultry Science Association, Inc. Environment and Health nationallicence drug residues nationallicence ampicillin nationallicence oxytetracycline nationallicence model nationallicence eggs nationallicence DONOGHUE DAN J. Pharmacology and Biochemistry Branch, Building 328-A, Agricultural Research Center, Center for Veterinary Medicine, Food and Drug Administration, Beltsville, Maryland 20705 aut HAIRSTON HERMAN Pharmacology and Biochemistry Branch, Building 328-A, Agricultural Research Center, Center for Veterinary Medicine, Food and Drug Administration, Beltsville, Maryland 20705 aut GAINES STUART A. Pharmacology and Biochemistry Branch, Building 328-A, Agricultural Research Center, Center for Veterinary Medicine, Food and Drug Administration, Beltsville, Maryland 20705 aut BARTHOLOMEW MARY J. Biometrics Branch, 7500 Standish Place, Center for Veterinary Medicine, Food and Drug Administration, Rockville, Maryland 20855 and aut DONOGHUE ANN M. Germplasm and Gamete Physiology Laboratory, Agricultural Research Service, USDA, Beltsville, Maryland 20705 aut Poultry Science Oxford University Press 75/3(1996-03), 321-328 0032-5791 75:3<321 1996 75 ps https://doi.org/10.3382/ps.0750321 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750321 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- DONOGHUE DAN J. Pharmacology and Biochemistry Branch, Building 328-A, Agricultural Research Center, Center for Veterinary Medicine, Food and Drug Administration, Beltsville, Maryland 20705 aut NATIONALLICENCE 700 1- HAIRSTON HERMAN Pharmacology and Biochemistry Branch, Building 328-A, Agricultural Research Center, Center for Veterinary Medicine, Food and Drug Administration, Beltsville, Maryland 20705 aut NATIONALLICENCE 700 1- GAINES STUART A. Pharmacology and Biochemistry Branch, Building 328-A, Agricultural Research Center, Center for Veterinary Medicine, Food and Drug Administration, Beltsville, Maryland 20705 aut NATIONALLICENCE 700 1- BARTHOLOMEW MARY J. Biometrics Branch, 7500 Standish Place, Center for Veterinary Medicine, Food and Drug Administration, Rockville, Maryland 20855 and aut NATIONALLICENCE 700 1- DONOGHUE ANN M. Germplasm and Gamete Physiology Laboratory, Agricultural Research Service, USDA, Beltsville, Maryland 20705 aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/3(1996-03), 321-328 0032-5791 75:3<321 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford