caa a22 4500 397567391 CHVBK 20180308164831.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.3382/ps.0751018 doi (NATIONALLICENCE)oxford-10.3382/ps.0751018 DNA Polymorphisms of Lipogenesis Genes and Analysis of Linkage with Fatness in Turkeys [Elektronische Daten] [M. SOURDIOUX, M. DOUAIRE, Y. DELABROSSE] DNA variability of lipogenesis genes was evaluated by Restriction Fragment Length Polymorphism (RFLP) on four selected lines of turkeys. Thirteen polymorphisms were obtained, four on the acetylcoenzyme A carboxylase gene, one on the apoprotein AI gene, two on the malic enzyme gene, four on the fatty acid synthase gene, and two on the stearoyl-coenzyme A desaturase gene. Six of these RFLP were studied in more detail on 64 female turkeys sampled in two lines. Large differences in terms of number of DNA profiles and haplotype frequencies were observed between the two lines, in accordance with the variability of recorded fatness performances. An additive model including the effects of RFLP profiles was used to assess linkage between polymorphisms on lipogenesis genes and fatness measured either in vivo by an ultrasonic instrument or, after slaughter, by the ratio of skin plus subcutaneous fat weight to the whole leg weight. In each line, two polymorphisms suggested a statistical association with fat deposit, one on the malic enzyme gene detected by DNA digestion with HindIII and one on the fatty acid synthase gene detected by MspI. A multiple comparison of means associated with each RFLP profile enabled the identification of two lean profiles in one strain, and a fat profile in the other. © 1996 Poultry Science Association, Inc. Molecular Biology nationallicence restriction fragment length polymorphism nationallicence turkey nationallicence fatness nationallicence lipogenesis genes nationallicence linkage analysis nationallicence SOURDIOUX M. Laboratoire de Génétique, Institut National de la Recherche Agronomique, Ecole Natinale Supérieure Agronomique de Rennes, 65 rue de Saint Brieuc, 35042 Rennes Cedex, France, and aut DOUAIRE M. Laboratoire de Génétique, Institut National de la Recherche Agronomique, Ecole Natinale Supérieure Agronomique de Rennes, 65 rue de Saint Brieuc, 35042 Rennes Cedex, France, and aut DELABROSSE Y. BETINA Sélection, Le Beau Chêne, Trédion, 56250 Elven, France aut Poultry Science Oxford University Press 75/8(1996-08), 1018-1026 0032-5791 75:8<1018 1996 75 ps https://doi.org/10.3382/ps.0751018 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0751018 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- SOURDIOUX M. Laboratoire de Génétique, Institut National de la Recherche Agronomique, Ecole Natinale Supérieure Agronomique de Rennes, 65 rue de Saint Brieuc, 35042 Rennes Cedex, France, and aut NATIONALLICENCE 700 1- DOUAIRE M. Laboratoire de Génétique, Institut National de la Recherche Agronomique, Ecole Natinale Supérieure Agronomique de Rennes, 65 rue de Saint Brieuc, 35042 Rennes Cedex, France, and aut NATIONALLICENCE 700 1- DELABROSSE Y. BETINA Sélection, Le Beau Chêne, Trédion, 56250 Elven, France aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/8(1996-08), 1018-1026 0032-5791 75:8<1018 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford