caa a22 4500 397567502 CHVBK 20180308164832.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.3382/ps.0750254 doi (NATIONALLICENCE)oxford-10.3382/ps.0750254 Cryopreservation of Rooster Semen in Thirteen and Sixteen Percent Glycerol [Elektronische Daten] [S.P.S. GILL, E. G. BUSS, R. J. MALLIS] Semen from Barred Plymouth Rock roosters was cryopreserved with glycerol concentrations of 13 and 16% in a microprocessor-controlled freezer. Thawing and deglycerolation were facilitated by the use of an improved microprocessor-controlled thawing device and high speed dialyzer. Deglycerolated semen (100 μL; 192 and 154 million sperm, respectively, for the 13 and 16% glycerol concentration) was inseminated into Single Comb White Leghorn hens. Three inseminations were done at 4-d intervals. Eggs were collected for 10 d starting 1 d after the first insemination, and incubated for 4 d. Fertility was determined by candling after the 4th d. Fertility measurements of 62.4 and 65% were obtained from the sperm frozen in 13 and 16% glycerol concentrations, respectively, for the 10-d period. © 1996 Poultry Science Association, Inc. Physiology and Reproduction nationallicence rooster nationallicence semen nationallicence cryopreservation nationallicence deglycerolation nationallicence insemination nationallicence GILL S.P.S. aut BUSS E. G. aut MALLIS R. J. aut Poultry Science Oxford University Press 75/2(1996-02), 254-256 0032-5791 75:2<254 1996 75 ps https://doi.org/10.3382/ps.0750254 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750254 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- GILL S.P.S. aut NATIONALLICENCE 700 1- BUSS E. G. aut NATIONALLICENCE 700 1- MALLIS R. J. aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/2(1996-02), 254-256 0032-5791 75:2<254 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford