caa a22 4500 39756757X CHVBK 20180308164832.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.3382/ps.0750579 doi (NATIONALLICENCE)oxford-10.3382/ps.0750579 Use of Randomly Amplified Polymorphic DNA Markers for the Genetic Analysis of Relatedness and Diversity in Chickens and Turkeys [Elektronische Daten] [E. J. SMITHS, C. P. JONES, J. BARTLETT, K. E. NESTOR] A study involving the use of random amplified polymorphic DNA (RAPD) was conducted to evaluate genetic polymorphism and relatedness within and among four chicken breeds: Araucona, Rhode Island Red, White Leghorn, and White Plymouth Rock, and two turkey populations, a long-term randombred and a commercial strain. A total of 60 random primers were used in the RAPD analyses. Forty-two of the 60 primers tested amplified patterns with at least one polymorphic fragment in one or more of the populations. Six of these 42 primers amplified polymorphic fragments in each of the six strains with a within- and between-population average band-sharing frequency of less than one but above zero (P < 0.05). Differences among the six primers for genetic distance (D) among populations were significant (P < 0.05). A consensus dendogram was therefore developed to show the phylogenetic relationships among the populations. As expected, estimates of D between populations were lowest within species and highest between species. The results provide evidence of the applicability of RAPD to determining genetic relatedness within and among different poultry populations and in developing reproducible markers useful in evaluating individual variation in chickens and turkeys. © 1996 Poultry Science Association, Inc. Breeding and Genetics nationallicence random amplified polymorphic DNA nationallicence genetic relatedness nationallicence polymorphism nationallicence chicken nationallicence turkey nationallicence SMITHS E. J. Department of Agricultural Sciences, Tuskegee University, Tuskegee, Alabama 36088 aut JONES C. P. Department of Agricultural Sciences, Tuskegee University, Tuskegee, Alabama 36088 aut BARTLETT J. Department of Agricultural Sciences, Tuskegee University, Tuskegee, Alabama 36088 aut NESTOR K. E. Department of Animal Science, Ohio Agricultural Research and Development Center, Ohio State University, Wooster, Ohio 44691 aut Poultry Science Oxford University Press 75/5(1996-05), 579-584 0032-5791 75:5<579 1996 75 ps https://doi.org/10.3382/ps.0750579 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750579 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- SMITHS E. J. Department of Agricultural Sciences, Tuskegee University, Tuskegee, Alabama 36088 aut NATIONALLICENCE 700 1- JONES C. P. Department of Agricultural Sciences, Tuskegee University, Tuskegee, Alabama 36088 aut NATIONALLICENCE 700 1- BARTLETT J. Department of Agricultural Sciences, Tuskegee University, Tuskegee, Alabama 36088 aut NATIONALLICENCE 700 1- NESTOR K. E. Department of Animal Science, Ohio Agricultural Research and Development Center, Ohio State University, Wooster, Ohio 44691 aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/5(1996-05), 579-584 0032-5791 75:5<579 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford