caa a22 4500 39756760X CHVBK 20180308164832.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.3382/ps.0750203 doi (NATIONALLICENCE)oxford-10.3382/ps.0750203 Differential Antibody Responses in 6.B Major Histocompatibility (B) Complex Congenic Chickens [Elektronische Daten] [MICHAEL C. DIX, ROBERT L. TAYLOR] Lines 6.6-2 (B 2 B 2) and 6.15-5 (B 2 B 2), congenic for the major histocompatibility (B) complex with > 99.9% background gene uniformity, were used to examine primary antibody responses to two antigens. In each of two trials, 1 mL of 5% SRBC, a T cell-dependent antigen, or 0.1 mL of Brucella abortus (BA), a T cell-independent antigen, was injected into separate groups of each B genotype aged 3 and 6 wk. Blood samples were taken from the chickens 7 d after immunization. Serum titers (log2) for both total antibody and mercaptoethanol (ME)-sensitive antibody to detect IgG were assayed by microtiter procedures. Least squares analysis of variance and Fisher's protected Least Significant Difference at P < 0.05 were used to evaluate the data. The total anti-SRBC antibody titer was significantly higher in B 5 B 5 chicks than in B 2 B 2 chicks at 4 and 7 w k of age. There was no significant difference in ME-sensitive antibody to SRBC. Chicks of the B 5 B 5 genotype also had significantly higher total and IgG antibody titers to BA at both ages than B 2 B 2 chicks. The results indicate that 4- and 7-wk-old B 5 B 5 chicks had a significantly stronger antibody response to SRBC or BA than B 2 B 2 chicks. © 1996 Poultry Science Association, Inc. Immunology nationallicence B complex nationallicence sheep red blood cells nationallicence Brucella abortus nationallicence DIX MICHAEL C. aut TAYLOR ROBERT L. 2To whom correspondence should be addressed. aut Poultry Science Oxford University Press 75/2(1996-02), 203-207 0032-5791 75:2<203 1996 75 ps https://doi.org/10.3382/ps.0750203 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750203 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- DIX MICHAEL C. aut NATIONALLICENCE 700 1- TAYLOR ROBERT L. 2To whom correspondence should be addressed aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/2(1996-02), 203-207 0032-5791 75:2<203 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford