caa a22 4500 397567723 CHVBK 20180308164832.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.3382/ps.0750186 doi (NATIONALLICENCE)oxford-10.3382/ps.0750186 Application of Direct-Fed Microbial Bacteria and Fructooligosaccharides for Salmonella Control in Broilers During Feed Withdrawal [Elektronische Daten] [OMAR A. OYARZABAL, DONALD E. CONNER] Providing direct-fed-microbial (DFM) bacteria and fructooligosaccharides (FOS) for the control of potential escalation of Salmonella colonization during simulated feed withdrawal and confinement was assessed. Eight hundred and eighty broilers (16 pens; 55 chicks per pen) were reared to 6 wk of age. Chicks were sprayed with a solution containing 106 nalidixic-acid resistant Salmonella typhimurium NR cells per milliliter on the 2nd d after hatching. Because this first challenge did not yield a high infection rate, chickens were rechallenged per os at Day 18 by providing water containing 107 cells of S. typhimurium NR per milliliter. At 3 and 5 wk of age, 10 birds per pen were euthanatized and cecal Salmonella were quantified (log colony-forming units per gram). Feed was removed from all pens at 6 wk, and pens were randomly assigned to be either the treatment group or the control group. The treatment groups were provided a DFM (mixture of nine bacteria) and FOS 50® (10%) in the drinking water. The control groups received drinking water only. After 6 h of feed withdrawal, chickens were cooped (eight per coop) and held 10 h. Immediately after confinement, 10 chickens were used for cecal enumeration of S. typhimurium NR . Salmonella colonization declined from 99% at 3 wk to 44% at 5 wk. After feed withdrawal, application of the treatment, and confinement, 11 and 14% of the treated and control groups, respectively, yielded S. typhimurium NR by direct plating from ceca (3.87 and 3.75 log10 cfu/g, respectively). No difference (P > 0.05) in Salmonella colonization occurred between the treated and the control groups; however, enrichment of ceca (incubation in nutrient broth at 37 C for 24 h) yielded a higher incidence of S. typhimurium NR in the control groups (32% in the treated vs 51% in the control). Ceca weights were greater in the treated group (P < 0.05). Simulated feed withdrawal and confinement did not escalate Salmonella colonization in the chicken ceca. © 1996 Poultry Science Association, Inc. Environment and Health nationallicence direct-fed microbial nationallicence competitive exclusion nationallicence fructooligosaccharides nationallicence Salmonella nationallicence OYARZABAL OMAR A. aut CONNER DONALD E. 2To whom correspondence should be addressed. aut Poultry Science Oxford University Press 75/2(1996-02), 186-190 0032-5791 75:2<186 1996 75 ps https://doi.org/10.3382/ps.0750186 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750186 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- OYARZABAL OMAR A. aut NATIONALLICENCE 700 1- CONNER DONALD E. 2To whom correspondence should be addressed aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/2(1996-02), 186-190 0032-5791 75:2<186 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford